羟基磷灰石陶瓷表面微沟槽的制备及其对成骨细胞早期行为的影响

doi:10.3724/SP.J.1077.2013.12093

摘要/Abstract

摘要：

利用超声探针在铝合金圆片上雕刻微米沟槽图案作为模板, 并成功地将此沟槽图案转移到羟基磷灰石(HAP)陶瓷表面上。扫描电子显微镜(SEM)表征结果显示3种沟槽宽度分别为20、40和60 μm, 与预先设计沟槽的尺寸一致; 接触角测量结果表明, 沟槽化HAP表面的纯水润湿性随沟槽宽度的减小而明显提高。对体外培养在三种沟槽尺寸HAP表面成骨细胞(MC3T3-E1)的形态观察、细胞核染色计数以及细胞取向角测量统计分析的结果表明, 沟槽化的HAP表面具有引导成骨细胞沿沟槽方向铺展的作用, 而且这种引导作用随着沟槽变窄而显著增强, 但这种引导作用并没有对成骨细胞的早期(30 h)增殖行为产生影响。

关键词: 羟基磷灰石陶瓷, 表面微沟槽, 微图案制备系统, 取向角

Abstract:

Hydroxyapatite (HAP) ceramic with orderly micro-patterned surface were fabricated by transferring micro-groove patterns from an aluminum alloy template, which was carved by an ultrasound-driven microprobe. The surface morphologies, phase compositions, and water wettability of samples were respectively analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), and contact angle measurements. The groove widths of three-kind samples were about 20, 40 and 60 μm, in lined with the designed values. Water wettability of the grooved HAP surfaces was found to be significantly increased by decreasing groove-width. Furthermore, the influences of the grooved HAP surfaces on the behavior of osteoblasts (MC3T3-E1) were examined by measuring cell orientation and counting the cell number. The results showed that the cells were oriented along the groove direction, and the cell orientation angles decreased with a decrease in groove-width, whereas no significant difference in the number of osteoblasts was observed. In summary, the groove-width of micro-patterned HAP has a distinct effect on the directed growth of osteoblast cells.

Key words: hydroxyapatite ceramics, surface micro-groove, micropatterned system, orientation angle

中图分类号:

TB321

王哲, 肖占文, 范红松, 张兴栋. 羟基磷灰石陶瓷表面微沟槽的制备及其对成骨细胞早期行为的影响[J]. 无机材料学报, 2013, 28(1): 51-57.

WANG Zhe, XIAO Zhan-Wen, FAN Hong-Song, ZHANG Xing-Dong. Fabrication of Micro-grooved Patterns on Hydroxyapatite Ceramics and Observation of Earlier Response of Osteoblasts to the Patterns[J]. Journal of Inorganic Materials, 2013, 28(1): 51-57.

图/表 9

图1 微图案超声加工系统

Fig. 1 Micropattern ultrasonic machining system

图2 HAP微图案制备过程示意图

Fig. 2 Schematic illustration of micropattern fabrication process of HAP

图3 图案化和未图案化的HAP表面SEM照片

Fig. 3 SEM images of topographical patterns of grooved (a, b and c) and unpatterned (d) HAP discs

Table 1 Sizes of groove patterns on HAP discs

Samples	Channel width/μm	Ridge width/μm	Ridge height/μm
HAP20	20.53±0.99	16.27±0.55	10.60±0.93
HAP40	37.29±3.05	24.30±2.40	9.61±1.14
HAP60	61.37±4.01	26.56±1.79	9.87±0.72

图4 沟槽化HAP表面的XRD图谱

Fig. 4 XRD pattern of a micro-grooved-HAP surface

图5 HAP沟槽宽度对水接触角的影响

Fig. 5 Dependence of the measured water contact angle on the groove width on HAP surfaces

图6 成骨细胞在HAP40表面培养12 h后的SEM照片

Fig. 6 SEM image of osteoblasts cultured for 12 h on the surface of HAP40

图7 成骨细胞在沟槽化和未沟槽化的HAP表面培养12 h后的细胞核的荧光照片和取向角统计直方图

Fig. 7 Fluorescent images of osteoblast nucleus (a-d) and histograms of osteoblast nucleus orientation angles (e-h)

图8 三种沟槽化和未沟槽化HAP表面的细胞数量(培养30 h)

Fig. 8 The number of osteoblasts cultured on smooth and grooved surfaces of HAP for 30 h

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