多孔硅酸钙生物陶瓷体内非骨性环境的植入研究

doi:10.3724/SP.J.1077.2008.00611

无机材料学报

多孔硅酸钙生物陶瓷体内非骨性环境的植入研究

许宋锋¹, 胡蕴玉¹, 林开利², 王臻¹, 王林¹, 常江², 白峰¹, 倪似愚³

1. 第四军医大学西京医院全军骨科研究所, 西安 710032; 2. 中国科学院上海硅酸盐研究所, 上海 200050; 3. 东华大学生物科学与技术研究所, 上海 201620

收稿日期:2007-06-25 修回日期:2007-09-22 出版日期:2008-05-20 网络出版日期:2008-05-20

In Vivo Study of Porous Calcium Silicate Bioceramic in Extra-osseous Sites

XU Song-Feng¹, HU Yun-Yu¹, LIN Kai-Li², WANG Zhen¹, WANG Lin¹, CHANG Jiang², BAI Feng¹, NI Si-Yu³

1. Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China; 2. Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China; 3. Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai 201620, China

Received:2007-06-25 Revised:2007-09-22 Published:2008-05-20 Online:2008-05-20

摘要/Abstract

摘要： 采用泡沫浸渍法制备了多孔硅酸钙(CS)和β-磷酸三钙(β-TCP)生物陶瓷材料, 将其植入家兔皮下筋膜组织中以研究其非骨性环境下的生物学行为. CS, β-TCP分别植入1、2、4周后取材, 采用SPECT、Micro-CT、V-G染色、SEM、EDX等方法进行样品的观察分析. 研究表明, 多孔硅酸钙生物陶瓷植入后未见明显毒性反应、且表面沉积了一层类骨羟基磷灰石层, 说明材料具有良好的生物相容性和生物活性. 植入4周时SPECT扫描表明, CS和β-TCP的ROI值分别为53.95±15.14和9.81±3.64(p<0.01), 表明CS的血管化程度明显高于β-TCP. 植入4周时Micro-CT分析表明, CS和β-TCP的残余材料占总体积百分比分别为(16.41±1.96)%和(30.72±0.69)%(p<0.05), 组织学半定量分析也表明, CS的残余面积明显小于β-TCP(p<0.01), 说明CS的降解性明显优于β-TCP. 与β-磷酸三钙相比较, 多孔硅酸钙陶瓷材料在早期血管化、新生组织形成、材料降解性方面均具有明显优势. 研究结果显示, 多孔硅酸钙生物陶瓷有望用作硬组织修复和组织工程用支架材料.

关键词: 多孔硅酸钙, 生物陶瓷, 动物实验, 降解性, 血管化

Abstract: Porous calcium silicate (CS) and β-tricalcium phosphate (β-TCP) bioceramics were obtained by sintering polymeric sponges infiltrated with ceramic slurry. They were implanted in rabbit subcutaneous sites and the biological characteristics were investigated. After 1, 2 and 4 weeks implantation, specimens were harvested and analyzed by SPECT, Von Gieson staining, Micro-CT, SEM and EDX. There is no obvious toxic reaction in porous CS ceramics, showing the excellent biocompatibility of CS ceramics. In SPECT scanning, the ROI of CS and β-TCP is (53.95±15.14) and (9.81±3.64), respectively (p<0.01), showing higher vascularization for CS. In Micro-CT analysis, the percentage of residual material volume fraction of CS and β-TCP after 4 weeks implantation is 16.41%±1.96% and 30.72%±0.69% respectively (p<0.01). In semi-quantitative analysis of histological observation, the percentage of residual material in CS is obviously lower than that in β-TCP. These results show that the biodegradation of CS is higher than that of β-TCP. The deposition of the bone-like hydroxyapatite layer after 2 week implantation show good bioactivity of CS in vivo. In conclusion, compared with β-TCP, porous CS bioceramics have superiority in vascularization, ingrowth of new tissue and degradation in early stage. Therefore, porous CS bioceramics may be potential candidates as biocompatible, bioactive and biodegradable scaffolds for hard tissue repair and tissue engineering applications.

Key words: porous calcium silicate, bioceramics, animal experiment, degradability, vascularization

中图分类号:

R318

许宋锋,胡蕴玉,林开利,王臻,王林,常江,白峰,倪似愚. 多孔硅酸钙生物陶瓷体内非骨性环境的植入研究[J]. 无机材料学报, DOI: 10.3724/SP.J.1077.2008.00611.

XU Song-Feng,HU Yun-Yu,LIN Kai-Li,WANG Zhen,WANG Lin,CHANG Jiang,BAI Feng,NI Si-Yu. In Vivo Study of Porous Calcium Silicate Bioceramic in Extra-osseous Sites[J]. Journal of Inorganic Materials, DOI: 10.3724/SP.J.1077.2008.00611.

参考文献

[1] De Aza P N, Luklinska Z B, Anseau M, et al. J. Microsc., 1996, 182 (Pt 1): 24--31.
[2] Tsuru K, Hayakawa S, Ohtsuki C, et al. J. Mater. Sci. Mater. Med., 1998, 9 (8): 479--484.
[3] Liu X, Ding C, Chu P K. Biomaterials, 2004, 25 (10): 1755--1761.
[4] 林开利, 常江, 汪正, 等(LIN Kai-Li, et al). 无机材料学报(Journal of Inorganic Materials), 2005, 20 (3): 692--698.
[5] De Aza P N, Luklinska Z B, Anseau M R, et al. J. Dent., 1999, 27 (2): 107--113.
[6] Sarmento C, Luklinska Z B, Brown L, et al. J. Biomed. Mater. Res. A, 2004, 69 (2): 351--358.
[7] Dufrane D, Delloye C, McKay I J, et al. J. Mater. Sci. Mater. Med., 2003, 14 (1): 33--38.
[8] Ni S, Chang J, Chou L, et al. J. Biomed. Mater. Res. B, 2007, 80 (1): 174--183.
[9] De Aza P N, Luklinska Z B, Martinez A, et al. J. Microsc, 2000, 197 (Pt 1): 60--67.
[10] De Aza P N, Luklinska Z B, Anseau M R, et al. J. Microsc, 2001, 201 (1): 33--43.
[11] Xue W, Liu X, Zheng X, et al. Biomaterials, 2005, 26 (17): 3455--3460.
[12] Giannoudis P V, Dinopoulos H, Tsiridis E. Injury, 2005, 36 (S1): S20--27.
[13] Patel Z S, Mikos A G. J. Biomater. Sci. Polym. Ed, 2004, 15 (6): 701--726.
[14] Smith L. Arch. Surg., 1963, 87: 653--661.
[15] Hulbert S F, Morrison S J, Klawitter J J. J. Biomed. Mater. Res., 1972, 6 (5): 347--374.
[16] Klawitter J J, Bagwell J G, Weinstein A M, et al. J. Biomed. Mater. Res., 1976, 10 (2): 311--323.
[17] Lin K L, Chang J, Zeng Y, et al. Mater. Lett., 2004, 58 (15): 2109--2113.
[18] Lin K L, Chang J, Lu J X, et al. Ceram. Int., 2007, 33 (4): 979--985.
[19] De Aza P N, De Aza A H, Herrera A, et al. J. Am. Ceram. Soc., 2006, 89 (8): 2619--2624.
[20] Dobrucki L W, Sinusas A J. Curr. Opin. Biotechnol., 2007, 18 (1): 90--96.
[21] van Lenthe G H, Hagenmuller H, Bohner M, et al. Biomaterials, 2007, 28 (15): 2479--2490.
[22] Ohsawa K, Neo M, Okamoto T, et al. J. Mater. Sci. Mater. Med., 2004, 15 (8): 859--864.
[23] Hench L L, Polak J M. Science, 2002, 295 (5557): 1014--1017.
[24] Ho S T, Hutmacher D W. Biomaterials, 2006, 27 (8): 1362--1376.

多孔硅酸钙生物陶瓷体内非骨性环境的植入研究

In Vivo Study of Porous Calcium Silicate Bioceramic in Extra-osseous Sites

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	陈梦杰, 王倩倩, 吴成铁, 黄健. 基于DFT的描述符预测生物陶瓷的降解性[J]. 无机材料学报, 2024, 39(10): 1175-1181.
[2]	郑嘉乾, 卢霄, 鲁亚杰, 王迎军, 王臻, 卢建熙. 医用生物陶瓷的功能性生物适配机制及应用[J]. 无机材料学报, 2024, 39(1): 1-16.
[3]	施哲, 刘伟业, 翟东, 谢建军, 朱钰方. 3D打印制备镁黄长石生物陶瓷骨组织工程支架及其性能[J]. 无机材料学报, 2023, 38(7): 763-770.
[4]	盛丽丽, 常江. 光/磁热Fe₂SiO₄/Fe₃O₄双相生物陶瓷及其复合电纺丝膜制备及抗菌性能研究[J]. 无机材料学报, 2022, 37(9): 983-990.
[5]	许宏一, 翟东, 曹琬婷, 陈振华, 钱文昊, 陈蕾. Li₂Ca₂Si₂O₇生物陶瓷的矿化活性研究[J]. 无机材料学报, 2021, 36(7): 753-760.
[6]	董少杰,王旭东,沈国芳,王晓虹,林开利. 生物陶瓷支架的功能改性及应用研究进展[J]. 无机材料学报, 2020, 35(8): 867-881.
[7]	刘子阳, 耿振, 李朝阳. 牡蛎壳为原料制备医用CaCO₃/HA复合生物材料[J]. 无机材料学报, 2020, 35(5): 601-607.
[8]	强小虎,李彬彬,黄大建,周松毅. 氧化硼对聚磷酸钙纤维力学和降解性能的影响[J]. 无机材料学报, 2019, 34(2): 201-206.
[9]	肖雯, 刘玉梅, 任凯歌, 匙峰, 李焰, 智伟, 翁杰, 屈树新. 利用鸡胚模型半体内评价多孔磷酸钙骨修复材料血管化的研究[J]. 无机材料学报, 2017, 32(6): 649-654.
[10]	杨永祝, 张雷, 杨国敬, 高长有, 苟中入. 多壳层化中空微球两相陶瓷生物材料制备研究[J]. 无机材料学报, 2014, 29(6): 650-656.
[11]	虞慧娴, 宁聪琴. CaSiO₃/TiO₂复合生物陶瓷的制备与体外性能研究[J]. 无机材料学报, 2013, 28(1): 69-73.
[12]	吴成铁, 常江. 硅酸盐生物活性陶瓷用于骨组织修复及再生的研究[J]. 无机材料学报, 2013, 28(1): 29-39.
[13]	刘福兴, 陈思翰. 生物陶瓷骨骼模型的制作[J]. 无机材料学报, 2012, 27(4): 348-352.
[14]	吴航, 张丽芳, 白威, 马驰, 熊成东. 聚合温度对聚磷酸钙聚合度及结构的影响[J]. 无机材料学报, 2012, 27(2): 174-178.
[15]	余静,赵荻,黄文旵,周萘,王德平,尹卫,陈亚青. 用作关节腔滑膜切除的放射性镝锂硼玻璃微球的性能研究[J]. 无机材料学报, 2010, 25(1): 58-62.