Immobilization of Lipase on KH560 Modified Silica by Sol-Gel Process

doi:10.15541/jim20150398

Abstract

Abstract:

An epoxy-functionalized silica support was prepared through 3-glycidoxypropyltrimethoxylsilane (KH560) modified silica sol in the presence of triblock copolymer F127 (F-560-S). Surface chemistry, micromorphology and pore structure of the supports were characterized by TG, FTIR, SEM and N₂ adsorption-desorption, which showed that the BET surface area and pore volume of epoxy-activated supports increased with the addition of F127. The Candida Antarctica Lipase B (CALB) was immobilized on the resulting carrier by covalent link. The amounts of lipases immobilized were 10 mg/g support and 375 mg/g support on the unmodified silica (U-S) and modified silica (F-560-S), respectively. The immobilized lipases were examined as biocatalysts for transesterification of 1-phenethanol and vinyl acetate in nonaqueous medium. The effects of solvents and temperature on immobilized lipases were systematically investigated. The catalytic activity of the CALB immobilized on F-560-S was improved in various solvents, especially in polar solvents. The immobilized CALBs maintained high activity, while the control experiment using free lipase gave very low ester production in the temperature range of 0-60 ℃. Furthermore, thermal stability of CALB immobilized on F-560-S was improved as compared to the CALB on U-S was observed. The CALB immobilized on F-560-S exhibited high operational stability in organic media which still retained 88.3% of its original activity for 12 h consecutive 7 runs.

Key words: KH560, F127, silica Sol-Gel, enzyme immobilization

CLC Number:

SONG Cong, YU Xiao-Wei, QIAN Dan, SUN Zhen-Zhong, JIANG Bo. Immobilization of Lipase on KH560 Modified Silica by Sol-Gel Process[J]. Journal of Inorganic Materials, 2016, 31(3): 311-316.

Figures/Tables 10

Fig. 1 TG curves of 560-S (A) and F-560-S (B)

Fig. 2 FT-IR spectra of silica before and after KH560 modificationA: KH560; B: U-S; C: F-560-S

Fig. 3 Low (a, c) and high (b, d) magnification SEM images of different silica support(a) and (b): U-S; (c) and (d): F-560-S

Table 1 Surface properties of silica support before and after modification

Sample	A_BET /(m²·g^-1)	Pore Volume/(cm³·g^-1)	Pore size/nm
U-S	538	0.563	3.9
560-S	455	0.545	3.2
F-560-S	725	0.709	10.3

Fig. 4 Lipase absorbed amount (mg/g support) of immobilized lipases on difference supports

Scheme 1 Transesterification of 1-phenylethanol and vinyl acetate

Fig. 5 Conversion of transesterification of 1-phenethanol and vinyl acetate using CALBs as biocatalysts(■) CALB immobilized on F-560-S; (□) CALB immobilized on 560-S; (●) CALB immobilized on U-S; (★) free CALB

Fig. 6 Reusability of the immobilized CALBs(■) CALB immobilized on F-560-S; (□) CALB immobilized on 560-S; (●) CALB immobilized on U-S

Fig. 7 Effect of solvents on transesterification of the immobilized CALBs

Fig. 8 Effect of temperature on transesterification of the immobilized CALBs(■) CALB immobilized on F-560-S; (□) CALB immobilized on 560-S; (●) CALB immobilized on U-S; (★) free CALB

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