Fabrication of Micro-grooved Patterns on Hydroxyapatite Ceramics and Observation of Earlier Response of Osteoblasts to the Patterns

doi:10.3724/SP.J.1077.2013.12093

Abstract

Abstract:

Hydroxyapatite (HAP) ceramic with orderly micro-patterned surface were fabricated by transferring micro-groove patterns from an aluminum alloy template, which was carved by an ultrasound-driven microprobe. The surface morphologies, phase compositions, and water wettability of samples were respectively analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), and contact angle measurements. The groove widths of three-kind samples were about 20, 40 and 60 μm, in lined with the designed values. Water wettability of the grooved HAP surfaces was found to be significantly increased by decreasing groove-width. Furthermore, the influences of the grooved HAP surfaces on the behavior of osteoblasts (MC3T3-E1) were examined by measuring cell orientation and counting the cell number. The results showed that the cells were oriented along the groove direction, and the cell orientation angles decreased with a decrease in groove-width, whereas no significant difference in the number of osteoblasts was observed. In summary, the groove-width of micro-patterned HAP has a distinct effect on the directed growth of osteoblast cells.

Key words: hydroxyapatite ceramics, surface micro-groove, micropatterned system, orientation angle

CLC Number:

TB321

WANG Zhe, XIAO Zhan-Wen, FAN Hong-Song, ZHANG Xing-Dong. Fabrication of Micro-grooved Patterns on Hydroxyapatite Ceramics and Observation of Earlier Response of Osteoblasts to the Patterns[J]. Journal of Inorganic Materials, 2013, 28(1): 51-57.

Figures/Tables 9

Fig. 1 Micropattern ultrasonic machining system

Fig. 2 Schematic illustration of micropattern fabrication process of HAP

Fig. 3 SEM images of topographical patterns of grooved (a, b and c) and unpatterned (d) HAP discs

Table 1 Sizes of groove patterns on HAP discs

Samples	Channel width/μm	Ridge width/μm	Ridge height/μm
HAP20	20.53±0.99	16.27±0.55	10.60±0.93
HAP40	37.29±3.05	24.30±2.40	9.61±1.14
HAP60	61.37±4.01	26.56±1.79	9.87±0.72

Fig. 4 XRD pattern of a micro-grooved-HAP surface

Fig. 5 Dependence of the measured water contact angle on the groove width on HAP surfaces

Fig. 6 SEM image of osteoblasts cultured for 12 h on the surface of HAP40

Fig. 7 Fluorescent images of osteoblast nucleus (a-d) and histograms of osteoblast nucleus orientation angles (e-h)

Fig. 8 The number of osteoblasts cultured on smooth and grooved surfaces of HAP for 30 h

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