Abstract: SiO₂ monoliths with double-pore structure were successfully synthesized with polyethylene glycol as mesopore-making agent and starch as macropore-making agent via a sol-gel route. The porous blocks, having mesopores with about 10nm pore size and macropores with 8~11μm pore size, could be formed from the removal of polyethylene glycol and starch after heated at 600℃. The minimal density of the obtained samples was 0.34g·cm^-3 and the maximal porosity was 76%. The original enzyme activity was improved from 5994U to 14702U by adding 30% of starch to the bare SiO₂ sample. After immersed in water at 80℃ for 7 days, the enzyme adsorption of the samples was increased and there was little difference between enzyme activity before and after immersion. Those results indicated that such double-pore materials with long-term stability on gluczyme immobilization should be easy to reclamation, reservation and recycle of enzyme.

Key words: double-pore structure, porous monolithic, sol-gel, enzyme carrier