磁性氧化铁高效磁共振造影剂的制备及应用

doi:10.15541/jim20140178

无机材料学报 ›› 2015, Vol. 30 ›› Issue (1): 53-58.DOI: 10.15541/jim20140178 CSTR: 32189.14.10.15541/jim20140178

磁性氧化铁高效磁共振造影剂的制备及应用

王军¹, 张宝林¹, 杨高¹, 王磊², 谢松伯¹, 李璇¹, 郜发宝²

(1. 桂林理工大学材料科学与工程学院, 广西有色金属及特色材料加工重点实验室, 省部共建国家重点实验室培育基地, 桂林 541004; 2. 四川大学华西医院放射科, 成都610041)

收稿日期:2014-04-08 修回日期:2014-06-20 出版日期:2015-01-20 网络出版日期:2014-12-29
作者简介:王军(1989–), 男, 硕士研究生. E-mail: wjs19892008@126.com
基金资助:
国家自然科学基金(51162003)

Synthesis and Application of Magnetic Iron Oxide Nanoparticles as High Efficiency Magnetic Resonance Imaging Contrast Agent

WANG Jun¹, ZHANG Bao-Lin¹, YANG Gao¹, WANG Lei², XIE Song-Bo¹, LI Xuan¹, GAO Fa-Bao²

(1. State Key Laboratory Breeding Base of Nonferrous Metals and Specific Materials Processing, School of Materials Science and Engineering, Guilin University of Technology, Guilin 541004, China; 2. Department of Radiology, West China Hospital, Sichuan University, Chengdu 610041, China)

Received:2014-04-08 Revised:2014-06-20 Published:2015-01-20 Online:2014-12-29
About author:WANG Jun. E-mail: wjs19892008@126.com
Supported by:
National Natural Science Foundation of China (51162003)

摘要/Abstract

摘要：

采用高温热分解法, 以乙酰丙酮铁为铁源, 生物相容性良好的聚乙二醇(PEG1000)作为溶剂、还原剂及修饰剂制备PEG修饰的氧化铁纳米粒子(PEG-SPIONs), 并研究其在小鼠体内的造影效果。X射线衍射(XRD)分析表明样品中含有Fe₃O₄晶相。透射电镜(TEM)结果显示, 合成的PEG-SPIONs形貌均一, 主要为等轴晶形, 纳米粒度及电位分析表明其表面呈负电性, 分散在水中的动力学粒径为20 nm。磁性能结果表明合成的PEG-SPIONs室温下具有超顺磁性, 并且具有较高的r₂/r₁值。细胞活性研究表明PEG-SPIONs具有较低的生物毒性, 体内的磁共振成像结果显示出PEG-SPIONs优异的对比增强效果, 说明PEG-SPIONs可以作为高效的T₂磁共振成像造影剂。

关键词: 氧化铁, 纳米粒子, 超顺磁性, 磁共振成像, 造影剂

Abstract:

Iron oxide nanoparticles were synthesized by thermal decomposition of iron (III) acetylacetonate (Fe(acac)₃) in poly(ethylene glycol) (PEG), which was used as the solvent, reducing agent and modifying agent. X-ray powder diffraction (XRD) analysis indicates that the nanoparticles have magnetite crystal structure. The results of transmission electron microscope (TEM) show that the shapes of iron oxide nanoparticles coated with PEG are equiaxial with uniform morphology. Particles and Zeta potential analyses show that the surface of iron oxide nanoparticles is negatively charged, and their hydrodynamics size dispersed in deionized water is 20 nm. Iron oxide nanoparticles show superparamagnetic behavior at room temperature and high r₂/r₁ratio. MTT studies show that PEG-SPIONs have low cytotoxicity. In vivo magnetic resonance imaging reveals their excellent contrast effects of the PEG-SPIONs. This work demonstrates that iron oxide nanoparticles coated with PEG can be excellent T₂ MRI contrast agents.

Key words: iron oxide, nanoparticles, superparamagnetic, magnetic resonance imaging (MRI), contrast agents

中图分类号:

O614

王军, 张宝林, 杨高, 王磊, 谢松伯, 李璇, 郜发宝. 磁性氧化铁高效磁共振造影剂的制备及应用[J]. 无机材料学报, 2015, 30(1): 53-58.

WANG Jun, ZHANG Bao-Lin, YANG Gao, WANG Lei, XIE Song-Bo, LI Xuan, GAO Fa-Bao. Synthesis and Application of Magnetic Iron Oxide Nanoparticles as High Efficiency Magnetic Resonance Imaging Contrast Agent[J]. Journal of Inorganic Materials, 2015, 30(1): 53-58.

图/表 9

图 1 采用高温热分解法在260℃反应1 h制备样品的XRD图谱

Fig. 1 XRD pattern of iron oxide nanoparticles prepared at 260℃ coated with PEG

图2 260℃ 制备的PEG-SPIONs的TEM照片及粒径分布图

Fig. 2 TEM image and size distribution of PEG-SPIONs prepared at 260℃

图 3 260℃ 制备的PEG-SPIONs的红外光谱图

Fig. 3 FTIR spectrum of PEG-SPIONs prepared at 260℃

图 4 在260℃制备的氧化铁纳米粒子在去离子水中的水合动力学粒径(a)与Zeta电位(b)

Fig. 4 Hydrodynamic size(a) and Zeta potential(b) of the iron oxide nanoparticles coated with PEG prepared at 260℃ in deionized water

图 5 PEG-SPIONs在300 K下的磁滞回线

Fig. 5 M-H curve of PEG-SPIONs at 300 K

图 6 MCF-7与不同浓度的PEG-SPIONs共同孵化24 h后的细胞存活率

Fig. 6 Cell viabilities of MCF-7 cells after incubation with different concentrations of PEG-SPIONs for 24 h

图 7 PEG-SPIONs在不同铁浓度下的加权成像(a)以及T2弛豫效率(b)

Fig. 7 T2 weight MR images (a) and the T2 relaxiation rate (b) of PEG-SPIONs with different Fe concentrations

图 8 尾静脉注射PEG-SPIONs前后小鼠脑部的T2*图像

Fig. 8 T2* MR images of the mouse brain before and after intravenous injection of PEG-SPIONs. (a) before injection, (b) 5 min, (c) 10 min, (d) 30 min, (e) 1 h, (f) 4 h and (g) 24 h after injection

图 9 尾静脉注射PEG-SPIONs前及注射24 h后的小鼠不同脑区的T2*值变化

Fig. 9 Relative T2* value of different brain regions extracted from T2* MR images of mouse brains before injection and 24 h after injection PEG-SPIONs

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磁性氧化铁高效磁共振造影剂的制备及应用

Synthesis and Application of Magnetic Iron Oxide Nanoparticles as High Efficiency Magnetic Resonance Imaging Contrast Agent

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