多次涂覆复合磷酸盐多孔陶瓷的表面结构和细胞相容性

doi:10.3724/SP.J.1077.2014.13268

无机材料学报 ›› 2014, Vol. 29 ›› Issue (2): 179-184.DOI: 10.3724/SP.J.1077.2014.13268 CSTR: 32189.14.SP.J.1077.2014.13268

多次涂覆复合磷酸盐多孔陶瓷的表面结构和细胞相容性

刘斌¹, 董寅生², 吴红艳¹, 苏静¹, 林萍华², 郭宗科³

(1. 南京信息工程大学物理与光电工程学院, 南京210044; 2. 东南大学材料科学与工程学院, 南京211189; 3. 东南大学附属中大医院, 南京210009)

收稿日期:2013-05-16 修回日期:2013-07-20 出版日期:2014-02-20 网络出版日期:2014-01-17
基金资助:
国家自然科学专项基金(51245010); 国家自然科学基金(51002079)

Surface Topology and Cytocompatibility of Polyphosphate Macroporous Ceramics with Recoating

LIU Bin¹, DONG Yin-Sheng², WU Hong-Yan¹, SU Jing¹, LIN Ping-Hua², GUO Zong-Ke³

(1. School of Physics and Optoelectronic Engineering, Nanjing University of Information & Technology, Nanjing 210044, China; 2. School of Materials Science and Engineering, Southeast University, Nanjing 211189, China; 3. Zhongda Hospital Southeast University, Nanjing 210009, China)

Received:2013-05-16 Revised:2013-07-20 Published:2014-02-20 Online:2014-01-17
Supported by:
National Natural Science Special Foundation of China (51245010); National Natural Science Foundation of China (51002079)

摘要/Abstract

摘要： 在制备复合磷酸盐多孔陶瓷的基础上, 采用稀浆料对其进行多次重复涂覆。通过扫描电镜(SEM)、排水法和体外细胞实验等对比了多次涂覆前后样品的孔隙特征, 表面结构和细胞相容性。结果发现, 随着涂覆次数的增加, 样品的孔壁厚度有所增加, 孔隙率呈下降趋势。不过经过3次涂覆后, 样品孔隙形貌并没有太大的变化, 孔隙率仍大于80%。同时多次涂覆使陶瓷表面增加了大量的1~2 μm的微孔结构, 体外实验结果显示, 这种结构有利于兔骨髓基质干细胞的粘附、铺展和生长。研究表明, 多次涂覆(特别是3次涂覆)在不明显改变多孔陶瓷孔隙特征的前提下, 改变了表面微观结构, 提高了细胞相容性。

关键词: 涂覆, 复合磷酸盐, 孔隙特征, 表面结构, 细胞相容性

Abstract: Polyphosphate porous ceramics were prepared and then recoated with thinner slurry. Macroporous characteristics, surface topology and cytocompatibility of samples before and after being recoated was evaluated using scan emission microscope (SEM), the Archimedes drainage method and the cells cultivation test in vitro. The results show that the pore wall thickness of samples increases with the increase of coating times, which slighty decreases their porosity. After being coated for 3 times, however, the macroporous morphology does not change obviously and the porosity is still more than 80%. Simultaneously, plenty of micropores with about 1–2 μm diameter are observed on the wall surfaces of samples. Furthermore, these surfaces are in favor of rabbit bone mesenchymal stem cells (RBMSC) adhesion, spreading and proliferation. These results indicate that the recoating process, especially repeated for 3 times, alters the surface topology of samples and enhances their cytocompatibility, but not sacrifices their macroporous characteristics.

Key words: recoating, polyphosphate, macroporous, surface topology, cytocompatibility

中图分类号:

TQ174
R318

刘斌, 董寅生, 吴红艳, 苏静, 林萍华, 郭宗科. 多次涂覆复合磷酸盐多孔陶瓷的表面结构和细胞相容性[J]. 无机材料学报, 2014, 29(2): 179-184.

LIU Bin, DONG Yin-Sheng, WU Hong-Yan, SU Jing, LIN Ping-Hua, GUO Zong-Ke. Surface Topology and Cytocompatibility of Polyphosphate Macroporous Ceramics with Recoating[J]. Journal of Inorganic Materials, 2014, 29(2): 179-184.

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多次涂覆复合磷酸盐多孔陶瓷的表面结构和细胞相容性

Surface Topology and Cytocompatibility of Polyphosphate Macroporous Ceramics with Recoating

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