葛根素/介孔生物活性玻璃复合材料的制备及体外协同促成骨作用研究

doi:10.15541/jim20250433

无机材料学报

• 研究论文 •

葛根素/介孔生物活性玻璃复合材料的制备及体外协同促成骨作用研究

徐铭^1,2, 王元非³, 吴桐^1,2

1.青岛大学纺织服装学院山东省医疗健康纺织材料重点实验室,青岛 266071;
2.青岛大学青岛大学附属医院医学研究中心,青岛 266000;
3.青岛大学附属青岛市口腔医院,青岛 266001

收稿日期:2025-10-30 修回日期:2025-11-24
通讯作者: 吴桐,教授. E-mail: tongwu24@163.com
作者简介:徐铭(1998-),男,博士研究生. E-mail: xumingmed@163.com
基金资助:
山东省自然科学基金（ZR2024MH163）,“纺织之光”中国纺织工业联合会应用基础研究项目（J202404）

Puerarin/Mesoporous Bioactive Glass Composites: Preparation and In Vitro Synergistic Osteogenic-promotion

XU Ming^1,2, WANG Yuanfei³, WU Tong^1,2

1. Shandong Key Laboratory of Medical and Health Textile Materials, College of Textiles and Clothing, Qingdao University, Qingdao 266071, China;
2. Medical Research Center, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266000, China;
3. Qingdao Stomatological Hospital Affiliated to Qingdao University, Qingdao 266001, China

Received:2025-10-30 Revised:2025-11-24
Contact: WU Tong, Professor. E-mail: tongwu24@163.com
About author:XU Ming (1998-), Male, PhD candidate. E-mail: xumingmed@163.com
Supported by:
Natural Science Foundation of Shandong (ZR2024MH163), “Textile Light” of China Textile Industry Federation Applied Basic Research Project (J202404)

摘要/Abstract

摘要： 由创伤、感染或骨质疏松引起的骨缺损是临床重大挑战,开发能够有效促进骨组织再生的生物活性材料尤为重要。而骨组织再生是一个极为复杂、受多因素影响的过程,现有骨生物材料均只关注有限因素的作用,迫切需要创制能协同促进成骨作用的新形复合材料。本研究通过溶胶-凝胶法制备了介孔生物活性玻璃（MBG）,并通过物理吸附方法构建了负载葛根素（Pue）的Pue/MBG复合材料。研究发现,所制备的MBG具有较高的比表面积和有序介孔结构,成功实现了Pue的负载和缓释。体外实验结果表明,Pue/MBG复合材料能有效促进小鼠胚胎前成骨细胞和大鼠骨髓间充质干细胞的增殖,同时增强其成骨分化能力,具体表现为,早期碱性磷酸酶活性的提高和晚期矿化结节形成的增强,并显示介孔生物活性玻璃与葛根素对成骨分化的协同作用。综上,本研究成功制备了一种基于Pue与MBG协同作用的高效骨修复复合材料,其良好的成骨能力在骨组织工程中具有良好应用前景。

关键词: 介孔生物活性玻璃, 葛根素, 成骨分化, 缓释, 生物相容性

Abstract: Bone defects caused by trauma, infection, or osteoporosis pose significant clinical challenges. Developing bioactive materials to effectively promote bone regeneration is particularly important. However, osteogenesis is a complex process influenced by multiple factors. Existing bone biomaterials only focus on the effects of limited factors, leading to an urgent need to develop novel composite materials that can synergistically promote osteogenesis. In this study, mesoporous bioactive glass (MBG) was prepared via sol-gel method, and puerarin (Pue)-loaded MBG composites (Pue/MBG) were constructed through physical adsorption. The results show that the prepared MBG exhibits high specific surface area and ordered mesoporous structure, successfully achieving Pue loading and sustained release. In vitro experiments demonstrate that Pue/MBG composites effectively promote the proliferation of mouse embryonic osteoblast precursor cells and bone mesenchymal stem cells, while enhancing their osteogenic differentiation capabilities. This is evidenced by increased early-stage alkaline phosphatase activity and enhanced late-stage mineralization nodule formation, showing synergistic effect of Pue and MBG. In conclusion, this study successfully develops an efficient bone-repair composite material based on the synergistic effect of Pue and MBG, exhibiting excellent osteogenic capacity and promising applications in bone tissue engineering.

Key words: mesoporous bioactive glass, puerarin, osteogenic differentiation, sustained-release, biocompatibility

中图分类号:

R318.08

徐铭, 王元非, 吴桐. 葛根素/介孔生物活性玻璃复合材料的制备及体外协同促成骨作用研究[J]. 无机材料学报, DOI: 10.15541/jim20250433.

XU Ming, WANG Yuanfei, WU Tong. Puerarin/Mesoporous Bioactive Glass Composites: Preparation and In Vitro Synergistic Osteogenic-promotion[J]. Journal of Inorganic Materials, DOI: 10.15541/jim20250433.

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葛根素/介孔生物活性玻璃复合材料的制备及体外协同促成骨作用研究

Puerarin/Mesoporous Bioactive Glass Composites: Preparation and In Vitro Synergistic Osteogenic-promotion

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