葛根素/介孔生物活性玻璃复合材料的制备及体外协同促成骨作用研究

doi:10.15541/jim20250433

无机材料学报 ›› 2026, Vol. 41 ›› Issue (7): 983-992.DOI: 10.15541/jim20250433

葛根素/介孔生物活性玻璃复合材料的制备及体外协同促成骨作用研究

徐铭¹^,²(), 王元非³, 吴桐¹^,²()

¹ 青岛大学纺织服装学院, 山东省医疗健康纺织材料重点实验室, 青岛 266071
² 青岛大学青岛大学附属医院医学研究中心, 青岛 266000
³ 青岛大学附属青岛市口腔医院, 青岛 266001

收稿日期:2025-10-30 修回日期:2025-11-24 出版日期:2026-07-20 网络出版日期:2026-01-21
通讯作者: 吴桐, 教授. E-mail: tongwu24@163.com
作者简介:徐铭(1998-), 男, 博士研究生. E-mail: xumingmed@163.com
基金资助:
山东省自然科学基金(ZR2024MH163);纺织之光应用基础研究计划(J202404)

Preparation and in vitro Synergistic Osteogenic-promoting Effect of Puerarin/Mesoporous Bioactive Glass Composites

XU Ming¹^,²(), WANG Yuanfei³, WU Tong¹^,²()

¹ Shandong Key Laboratory of Medical and Health Textile Materials, College of Textiles and Clothing, Qingdao University, Qingdao 266071, China
² Medical Research Center of Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266000, China
³ Qingdao Stomatological Hospital Affiliated to Qingdao University, Qingdao 266001, China

Received:2025-10-30 Revised:2025-11-24 Published:2026-07-20 Online:2026-01-21
Contact: WU Tong, professor. E-mail: tongwu24@163.com
About author:XU Ming (1998-), male, PhD candidate. E-mail: xumingmed@163.com
Supported by:
Natural Science Foundation of Shandong(ZR2024MH163);Textile Vision Basic Research Program of China National Textile and Apparel Council(J202404)

摘要/Abstract

摘要：

由创伤、感染或骨质疏松引起的骨缺损是临床重大挑战, 开发能够有效促进骨再生的生物活性材料尤为重要。本研究通过溶胶-凝胶法制备了介孔生物活性玻璃(MBG), 并通过物理吸附法构建了负载葛根素(Pue)的Pue/MBG复合材料。结果显示, 制备的MBG具有较大比表面积和有序介孔结构, 成功实现了Pue的负载和缓释。体外实验结果表明, Pue/MBG复合材料能有效促进小鼠胚胎前成骨细胞和大鼠骨髓间充质干细胞的增殖, 同时增强其成骨分化能力, 具体表现为早期碱性磷酸酶活性的提高和晚期矿化结节形成的增强。综上, 本研究成功制备了一种基于Pue与MBG协同作用的高效骨修复复合材料, 其良好的成骨能力在骨组织工程中具有良好的应用前景。

关键词: 介孔生物活性玻璃, 葛根素, 成骨分化, 缓释, 生物相容性

Abstract:

Bone defects caused by trauma, infection, or osteoporosis pose significant clinical challenges. Developing bioactive materials that effectively promote bone regeneration is particularly important. In this study, mesoporous bioactive glass (MBG) was prepared via the Sol-Gel method, and puerarin (Pue)-loaded MBG (Pue/MBG) composites were constructed through physical adsorption. The results show that the prepared MBG exhibits a high specific surface area and an ordered mesoporous structure, successfully achieving Pue loading and sustained release. In vitro experiments demonstrate that Pue/MBG composites effectively promote the proliferation of mouse embryonic osteoblast precursor cells and rat bone marrow mesenchymal stem cells, while enhancing their osteogenic differentiation capabilities. This is evidenced by increased early-stage alkaline phosphatase activity and enhanced late-stage mineralization nodule formation. In conclusion, this study successfully develops an efficient bone-repair composite material based on the synergistic effect of Pue and MBG, with its excellent osteogenic capacity showing promising applications in bone tissue engineering.

Key words: mesoporous bioactive glass, puerarin, osteogenic differentiation, sustained-release, biocompatibility

中图分类号:

R318

徐铭, 王元非, 吴桐. 葛根素/介孔生物活性玻璃复合材料的制备及体外协同促成骨作用研究[J]. 无机材料学报, 2026, 41(7): 983-992.

XU Ming, WANG Yuanfei, WU Tong. Preparation and in vitro Synergistic Osteogenic-promoting Effect of Puerarin/Mesoporous Bioactive Glass Composites[J]. Journal of Inorganic Materials, 2026, 41(7): 983-992.

图/表 11

图1 Pue/MBG复合材料的制备及促成骨作用示意图

Fig. 1 Preparation and osteogenic-promoting mechanism of Pue/MBG composites RT: room temperature

图2 MBG和Pue/MBG的表面形貌和粒径分析

Fig. 2 Surface morphology and particle size analysis of MBG and Pue/MBG (a, b) Digital photos of (a) MBG and (b) Pue/MBG; (c, d) SEM images of (c) MBG and (d) Pue/MBG; (e, f) TEM images of (e) MBG and (f) Pue/MBG; (g, h) Particle size distribution diagrams of (g) MBG and (h) Pue/MBG

图3 MBG和Pue/MBG的(a) N2吸附-脱附等温线以及(b)对应的孔径分布

Fig. 3 (a) N2 adsorption-desorption isotherms and (b) corresponding pore size distributions of MBG and Pue/MBG

表1 MBG和Pue/MBG的比表面积与介孔分析

Table 1 Specific surface area and mesoporous analysis of MBG and Pue/MBG

Sample	BET surface area/(m²·g⁻¹)	Pore volume/ (cm³·g⁻¹)	BJH pore size/nm
MBG	373.28	0.70	12.93
Pue/MBG	151.02	0.45	8.84

图4 MBG和Pue/MBG的XRD谱图

Fig. 4 XRD patterns of MBG and Pue/MBG (a) Wide-angle XRD pattern of MBG; (b) Small-angle XRD patterns of MBG and Pue/MBG; (c) Enlarged view of rectangular region in Fig. (b)

图5 (a, b) MBG和(c, d) Pue/MBG的EDS分析

Fig. 5 EDS analysis of (a, b) MBG and (c, d) Pue/MBG (a, c) EDS elemental mappings; (b, d) EDS point spectra

图6 Pue、MBG和Pue/MBG的FT-IR图谱

Fig. 6 FT-IR spectra of Pue, MBG and Pue/MBG

表2 Pue/MBG的载药量、载药率和包封率

Table 2 Drug loading capacity, drug loading rate, and encapsulation efficiency of Pue/MBG

Sample	DLC/μg	DLR/%	EE/%
Pue/MBG	338.38±16.05	33.84±1.61	9.02±0.43

图7 Pue/MBG的药物释放曲线

Fig. 7 Drug release curve of Pue/MBG

图8 Pue/MBG的体外生物相容性评估

Fig. 8 In vitro biocompatibility evaluation of Pue/MBG (a, c) Cell viability assay of (a) MC3T3-E1 and (c) rBMSCs; (b, d) Cell morphology staining of (b) MC3T3-E1 and (d) rBMSCs. Colorful figures are available on website

图9 Pue/MBG的成骨分化作用

Fig. 9 Osteogenic differentiation effects of Pue/MBG (a) ALP activity assay and (b) ALP staining images on 7 days; (c) ARS quantitative analysis and (d) ARS staining images on 14 days. Colorful figures are available on website

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葛根素/介孔生物活性玻璃复合材料的制备及体外协同促成骨作用研究

Preparation and in vitro Synergistic Osteogenic-promoting Effect of Puerarin/Mesoporous Bioactive Glass Composites

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