Adv. Search
Journal of Inorganic Materials

Journal of Inorganic Materials >

2006 , Vol. 21 >Issue 5: 1154 - 1160

DOI: https://doi.org/10.3724/SP.J.1077.2006.01154

Research Paper

Synthesis and Enzyme Activity of SiO2 Monolithic Carrier with Double-pore Structure by Addition of Starch/PEG

  • YANG Hong-Bin ,
  • CHEN Qi ,
  • SONG Li ,
  • YE Rei-Fang ,
  • HOU Feng-Zhen ,
  • LU Jian-Ying
Expand
  • 1. Department of Inorganic Materials, East China University of Science and Technology, Shanghai 200237, China; 2. Department of Biochemistry Engineering, East China University of Science and Technology, Shanghai 200237, China)

Received date: 2005-10-21

  Revised date: 2005-12-22

  Online published: 2006-09-20

Fold

Abstract

SiO2 monoliths with double-pore structure were successfully synthesized with polyethylene glycol as mesopore-making agent and starch as macropore-making agent via a sol-gel route. The porous blocks, having mesopores with about 10nm pore size and macropores with 8~11μm pore size, could be formed from the removal of polyethylene glycol and starch after heated at 600℃. The minimal density of the obtained samples was 0.34g·cm-3 and the maximal porosity was 76%. The original enzyme activity was improved from 5994U to 14702U by adding 30% of starch to the bare SiO2 sample. After immersed in water at 80℃ for 7 days, the enzyme adsorption of the samples was increased and there was little difference between enzyme activity before and after immersion. Those results indicated that such double-pore materials with long-term stability on gluczyme immobilization should be easy to reclamation, reservation and recycle of enzyme.

Key words： double-pore structure; porous monolithic; sol-gel; enzyme carrier

Cite this article

YANG Hong-Bin , CHEN Qi , SONG Li , YE Rei-Fang , HOU Feng-Zhen , LU Jian-Ying . Synthesis and Enzyme Activity of SiO2 Monolithic Carrier with Double-pore Structure by Addition of Starch/PEG[J]. Journal of Inorganic Materials, 2006 , 21(5) : 1154 -1160 . DOI: 10.3724/SP.J.1077.2006.01154

References

[1] Lei J, Fan J, Yu C-Z, et al. Microporous. Mesoporous. Mater., 2004, 73: 121--128.
[2] Sayari Abdelhamid, Hamoudi Safia, Yang Yong. Chem. Mater., 2005, 17: 212--216.
[3] Costela A, Moreno Garcia I, Gomez C, et al. J. Phys. Chem. B., 2005, 109: 4475--4480.
[4] Davis Mark E. Nature., 2002, 417 (6891): 813--821.
[5] Trong On Do, Kaliaguine. J. Am. Chem. Soc., 2003, 125: 618--619.
[6] Izabela Nowak. Colloids and Surfaces A: Physicochem. Eng. Aspects., 2004, 241: 103--111.
[7] Gomez J M, Deere J, Goradia D, et al. Catal. Lett., 2003, 88: 183--186.
[8] Pandya P H, Iasra R V, Newalkar B L, et al. Microporous. Mesoporous. Mater., 2005, 77: 67--77.
[9] 汪秀全, 杨洪斌, 陈奇, 等(Wang Xiu-Quan, et al). 硅酸盐学报(Journal of the Chinese Ceramic Society), 2005, 33 (8): 975--979.
[10] 刘海荣, 陈奇(LIU Hai-Rong, et al). 硅酸盐学报(Journal of the Chinese Ceramic Society), 2005, 33 (4): 438--442.
[11] 胡仁其, 于家波, 宋富兵, 等. 化学学报, 2000, 58 (10): 1211--1215.
[12] 雷杰, 余承忠, 范杰, 等. 化学学报, 2005, 63 (8): 739--744.
[13] 徐如人, 庞文琴. 分子筛与多孔材料化学, 第一版. 科学出版社, 2004. 145--148.

Options
Outlines

/

〈 〉