Study of Fibroblast and Platelet Adhesion on Multi-wallCarbon Nanotubes

doi:10.3724/SP.J.1077.2011.00073

Journal of Inorganic Materials ›› 2011, Vol. 26 ›› Issue (1): 73-77.DOI: 10.3724/SP.J.1077.2011.00073

• Research Paper • Previous Articles Next Articles

Study of Fibroblast and Platelet Adhesion on Multi-wallCarbon Nanotubes

ZHAO Meng-Li¹, YUE Yu-Chen¹, YUAN Li¹,LI De-Jun¹, LÜ Xiao-Ying², HUANG Yan²

1. College of Physics and Electronic Information Science, Tianjin NormalUniversity, Tianjin 300387, China; 2. State Key Laboratory ofBioelectronics, Southeast University, Nanjing 210096, China

Received:2010-04-28 Revised:2010-06-05 Published:2011-01-20 Online:2010-12-23
Supported by:
National Natural Science Foundation ofChina(11075116); Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University(2010-2012); Key Laboratory of Beam Technology and Material Modification of Ministry of Education, Beijing Normal University(201014)

Abstract

Abstract: Abstract:Multi-walled nanotubes (MWCNTs) were grown on carbonpapers by chemical vapor deposition (CVD). Adhesion and proliferation of L929 mouse fibroblasts and plateletson MWCNTs and carbon papers were investigated. The influence of different bloodprotein adsorption on the human skin fibroblasts growth was also studied. The resultsshowed that mouse fibroblasts implanted on MWCNTs tended to grow more prolificallythan those on carbon papers. The cell concentration observed on MWCNTsincreased from 1.25´10⁵/mL (cultured for 1 d) to 4.1´10⁵/mL (culture for 7 d). No toxicity reaction was observed during the culturingperiod.By employing the adsorptionassay, it was found albumin, fibrinogen, and immunoglobulinG adhered on materials could promotethe adhesion and growth of the human skin fibroblasts. Furthermore, plateletadhesion rate on MWCNTs is lower than that on the carbon papers. These resultsindicate that MWCNTs have better tissue compatibility and blood compatibility.

Key words: multi-wall carbon nanotubes, cell adhesion, proteinadsorption, platelet adhesion

CLC Number:

TB34
Q819

ZHAO Meng-Li, YUE Yu-Chen, YUAN Li,LI De-Jun, LÜ Xiao-Ying, HUANG Yan. Study of Fibroblast and Platelet Adhesion on Multi-wallCarbon Nanotubes[J]. Journal of Inorganic Materials, 2011, 26(1): 73-77.

References

[1] Sander C G L, John A J, Jos M, et al. Trends in biomaterials research: an analysis of the scientific program of the World Biomaterials Congress 2008. Biomaterials, 2008, 29(21): 3047-3052.

[2] Chor Y T, Gu H G, Leong W S, et al. Cellular behavior of human mesenchymal stem cells cultured on single-walled carbon nanotube film. Carbon, 2010, 48(4): 1095-1104.

[3] Eva H, Alan C, Fiona M L, et al. A new approach to the toxicity testing of carbon-based nanomaterials-the clonogenic assay. Toxicology Letters, 2007, 174(1/2/3): 49-60.

[4] Karin P, Jorg M W, Frank H, et al. Human lung epithelial cells show biphasic oxidative burst after single-walled carbon nanotube contact. Carbon, 2007, 45(11): 2241-2249.

[5] Xiao C W, Zhang W J, Ramaswami S, et al. Non-functionalized carbon nanotube binding with hemoglobin. Colloids and Surfaces B: Biointerfaces, 2008, 65(1): 146-149.

[6] Alla L A, Shan W Q, Pavel B, et al. Single-walled carbon nanotubes dispersed in aqueous media via non-covalent functionalization: effect of dispersant on the stability, cytotoxicity, and epigenetic toxicity of nanotube suspensions. Water Research, 2010, 44(2): 505-520.

[7] Laura E V, Pablo A F, Carlos D G, et al. The adsorption-desorption process of bovine serum albumin on carbon nanotubes. Journal of Colloid and Interface Science, 2007, 307(2): 349-356.

[8] Curtis A, Wikinsion C. Topographical control of cells. Biomaterials, 1997, 18(24): 1573-1583.

[9] Kenichi M, Frank X W, Masao Y, et al. The attachment and growth behavior of osteoblast-like cells on microtextured surfaces. Biomaterials, 2003, 24(16): 2711-2719.

[10] Puja C, Ajit S, Anju S. Cisplatin primes murine peritoneal macrophages for enhanced expression of nitric oxide, proinflammatory cytokines, TLRs, transcription factors and activation of MAP kinases upon co-incubation with L929 cells. Immunobiology, 2009, 214(3): 197-209.

[11] Van K T G, Spijker H T, Busscher H J. Plasma-treated polystyrene surfaces: model surfaces for studying cell-biomaterial interactions. Biomaterials, 2004, 25(10): 1735-1747.

Study of Fibroblast and Platelet Adhesion on Multi-wallCarbon Nanotubes

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