Journal of Inorganic Materials ›› 2022, Vol. 37 ›› Issue (5): 554-560.DOI: 10.15541/jim20210365

• RESEARCH ARTICLE • Previous Articles     Next Articles

Ruthenium-biocarbon Mimic Enzyme: Synthesis and Application in Colorimetric Detection of Pesticide Chlorpyrifos

CAO Zhijun(), LI Zaijun()   

  1. School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China
  • Received:2021-06-06 Revised:2021-08-07 Published:2022-05-20 Online:2021-11-01
  • Contact: LI Zaijun, professor. E-mail:
  • About author:CAO Zhijun (1991-), male, PhD candidate. E-mail:
  • Supported by:
    Research and Development Program of China(2018YFC1603001)


Metal nanomaterials as mimic enzyme have been widely used for biosensor and catalysis due to their high stability and low cost. Here, [1-methyl-3-ethylimidazole][dicyandiamide] ionic liquid ([EMIM][DCA]) reacted with ruthenium chloride to form [EMIM]3[Ru(DCA)6] ionic liquid. Silk was dissolved in the above ionic liquid and then carbonized at high temperature to obtain Ru-biomass carbon composite. The results show that Ru-biomass carbon offers excellent dispersion, small Ru nanoparticles with an average diameter of 7.5 nm which were uniformly dispersed on the surface of carbon sheets, and high oxidase-like activity. Based on the inhibition of chlorpyrifos on catalytic activity of Ru-biomass carbon, a method was established for colorimetric determination of chlorpyrifos. Chlorpyrifos obviously inhibits the catalytic activity of Ru-biochar carbon, leading to a reduced absorbance of blue product. When the concentration of chlorpyrifos was in the range from 10 to 80 ng/mL, the peak absorbance decreases linearly with the increase of chlorpyrifos concentration, giving a detection limit of about 6.5 ng/mL. The as-proposed method provides higher sensitivity and stability compared with present methods, and has successfully applied to detection of chlorpyrifos in peach samples.

Key words: metal ionic liquid, Ru nanomaterial, oxidase-like enzyme, organophosphorus insecticides, colorimetric detection

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