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2007 , Vol. 22 >Issue 5: 996 - 1000

DOI: https://doi.org/10.3724/SP.J.1077.2007.00996

研究论文

Ti-O-N表面肝素分子的共价固定及抗凝血性研究

  • 侯瑞霞 ,
  • 翁亚军 ,
  • 王进 ,
  • 王志浩 ,
  • 冷永祥 ,
  • 黄楠
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  • 西南交通大学材料先进技术教育部重点实验室, 人工器官表面工程四川省重点实验室, 成都 610031

收稿日期: 2006-09-30

  修回日期: 2006-11-24

  网络出版日期: 2007-09-20

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Study on Covalent Immobilization of Heparin on Ti-O-N Surface and Antithrombogenicity

  • HOU Rui-Xia ,
  • WENG Ya-Jun ,
  • WANG Jin ,
  • WANG Zhi-Hao ,
  • LENG Yong-Xiang ,
  • HUANG Nan
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  • Key Lab. for Advanced Technologies of Materials of Ministry of Education; Lab of Artificial Organs and Surface Engineering, Southwest Jiaotong University, Chengdu 610031, China

Received date: 2006-09-30

  Revised date: 2006-11-24

  Online published: 2007-09-20

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摘要

利用磷酸化学吸附方法扩增Ti-O-N薄膜表面的羟基, 扩大与3-氨丙基三乙氧基硅烷(APTES)化学反应的位点, 进而增加Ti-O-N薄膜表面固定的肝素量. 荧光染色法定性分析证明了APTES末端氨基的存在, 甲苯胺蓝法定量测定H3PO4处理后的Ti-O-N表面肝素浓度为6.6μg/cm2. 体外血小板粘附实验表明, 经磷酸处理并固定肝素的Ti-O-N膜表面能够有效抑制血小板的粘附和变形, 具有良好的抗凝血性能. 这为制备无机材料的抗凝血表面构建提供了一个有效的技术手段.

关键词： Ti-O-N薄膜; 磷酸化学吸附; 共价固定肝素; 抗凝血性

本文引用格式

侯瑞霞 , 翁亚军 , 王进 , 王志浩 , 冷永祥 , 黄楠 . Ti-O-N表面肝素分子的共价固定及抗凝血性研究[J]. 无机材料学报, 2007 , 22(5) : 996 -1000 . DOI: 10.3724/SP.J.1077.2007.00996

Abstract

Fourier transform infrared spectroscope (FTIR) and X-ray photoelectron spectroscope (XPS) were used to characterize the surface component and bonding state, and these analyses show that the content of hydroxyl group on the surface of Ti-O-N film is enhanced by the chemisorption of phosphoric acid, and the reaction sites with 3-aminopropyltriethoxysilane (APTES) are amplified, furthermore the concentration of immobilized heparin molecules are increased. Fluorescence staining analysis qualitatively proves that the terminal amino groups of APTES are in existence. The result of the toluidine blue method shows that the concentrations of the immobilized heparin on the Ti-O-N film treated by phosphoric acid are 6.6μg/cm2. The evaluation of the platelet adhesion test in ~vitro indicates that the Ti-O-N film treated by phosphoric acid and then immobilized with the heparin molecules can effectively suppress the adhesion and activation of the platelets, and has a better antithrombotic property.

Key words： Ti-O-N film; chemisorption of phosphate; covalent immobilization of heparin; antithrombogenicity

参考文献

[1] Huang N, Yang P, Leng Y X, et al. Biomaterials, 2003, 23: 2177--2187.
[2] Yang P, Huang N, Leng Y X, et al. Biomaterials, 2003, 24: 2821--2829.
[3] Thomas C, Gladwin S D, Robert F W, et al. Biomaterials, 2000, 21: 699--712.
[4] Philip R B, Elijah W M, Elodie M M, et al. Thromb. Res., 2000, 98: 249--256.
[5] Chen Song-Zhe, Zhang Ping-Yi, Zhuang Da-Ming, et al. Catalysis Communications, 2004, 5: 677--680.
[6] 万国江, 黄楠, 冷永祥,等(WAN Guo-Jian, et al). 无机材料学报(Journal of Inorganic Materials), 2003, 18 (4): 904--910.
[7] 李艳红, 曹文斌, 韦祎,等. 无机材料学报(Journal of Inorganic Materials), 2006, 21 (5): 1067--1072.
[8] Ellen S G, Kristina B R, Mark S D, et al. Langmuir, 2001, 17: 6743--6745.
[9] 刘建伟, 陈元维, 唐昌伟, 等. 四川大学学报, 2004, 36 (1): 41--44.
[10] Joseph I Z, Claire G, Peter H, et al. J. Surg. Res., 1997, 67: 4--8.
[11] Philip R D, Nicholas G N. Appl. Surf. Sci., 2001, 181: 296--306.
[12] Chen Y X, Liu W M, Ye C F, et al. Materials Research Bulletin, 2001, 36: 2605--2612.
[13] 刘敬肖, 杨大智, 蔡英骥. 材料研究学报, 2002, 16 (5): 529--535.
[14] 姚超, 高国生, 林西平, 等(YAO Chao, et al). 无机材料学报(Journal of Inorganic Materials), 2006, 21 (2): 315--321.
[15] 王忠东, 王玉田. 光谱学与光谱分析, 2005, 25 (10): 1645--1647.
[16] 王琴梅, 潘仕荣, 郑振声. 北京生物医学工程, 1997, 16 (1): 44--49.
[17] Kristensen E M E, Larsson R, Sanchez J, et al. Colloids and Surfaces B: Biointerfaces, 2006, 49: 1--7.
[18] Denis L, Christine V, Cedric C, et al. Biomaterials, 2005, 26: 5075--5084.
[19] 王琴梅, 潘仕荣, 郑振声. 功能材料, 1997, 28 (6): 644--647.
[20] Yali L, Neoh K G, Kang E T. Polymer, 2004, 45: 8779--8789.
[21] 邱永兴, 胡勇, 计剑, 等. 高分子学报, 1997, (3): 263--270.
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