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无机材料学报

无机材料学报 ›› 2011, Vol. 26 ›› Issue (12): 1299-1303.DOI: 10.3724/SP.J.1077.2011.01299 CSTR: 32189.14.SP.J.1077.2011.01299

• 研究论文 • 上一篇    下一篇

BSA和FN在纳米化钛表面的蛋白吸附及释放行为

史 婕, 冯 波, 鲁 雄, 汪建新, 段 可, 翁 杰   

  1. (西南交通大学 材料先进技术教育部重点实验室, 成都 610031)
  • 收稿日期:2011-01-01 修回日期:2011-03-18 出版日期:2011-12-20 网络出版日期:2011-11-11
  • 作者简介:史 婕(1986-), 女, 硕士研究生. E-mail: shilinyue98@126.com
  • 基金资助:

    国家自然科学基金(50871093); 教育部高等学校全国优秀博士学位论文作者专项基金(200554)

Adsorption and Release Behavior of BSA and FN on Nanostructural Ti Surface

SHI Jie, FENG Bo, LU Xiong, WANG Jian-Xin, DUAN Ke, WENG Jie   

  1. (Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China)
  • Received:2011-01-01 Revised:2011-03-18 Published:2011-12-20 Online:2011-11-11
  • About author:SHI Jie. E-mail: shilinyue98@126.com
  • Supported by:

    National Natural Science Foundation of China (50871093); Foundation for the Author of National Excellent Doctoral Dissertation of China (200554)

PDF (PC)

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被引次数

13 | 1

摘要： 通过阳极氧化技术, 在钛表面制备一层管径为100nm左右的氧化钛纳米管. 选取小牛血清白蛋白(BSA)和纤维连接蛋白(FN)两种蛋白质进行蛋白吸附实验, 并在仿生条件下进行蛋白质的体外释放. 对吸附了蛋白的纳米管试样表面进行红外和荧光定性分析, 同时采用考马斯亮蓝法对纳米管表面的蛋白吸附进行定量检测, 实验发现纳米管试样更有利于蛋白质的吸附, 且FN在试样表面吸附时的吸附率大于BSA. 氧化钛纳米管的蛋白释放分为突释和缓释两个阶段, 其释放机制符合Fickian扩散.

关键词: 氧化钛纳米管, 蛋白质吸附及释放, 小牛血清白蛋白, 纤维连接蛋白

Abstract: The titanium dioxide (TiO2) nanotube arrays with 100 nm diameters on titanium were fabricated by anodic oxidation. The adsorption behaviors of bovine serum albumin (BSA) and fibronectin (FN) onto the nanotube arrays were investigated, and then the protein releases were examined in vitro. Fourier transform infrared spectroscope and Fluorescence microscope were used to qualitative analysis for TiO2 nanotube arrays surfaces after protein adsorption. Adsorbance of protein was detected with the method of Coomassie brilliant blue G-250. The results showed that TiO2 nanotube arrays could obviously improve the adsorption, and the adsorption percentage of FN on samples was larger than that of BSA. The release experiment results suggested that the release behaviors of BSA and FN on the nanotube arrays involved the bust release and the gradual release, and the release mechanism was consistent with Fickian diffusion.

Key words: TiO2 nanotube, protein adsorption and release, bovine serum albumin, fibronectin

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