采用水热法制备了纳米羟基磷灰石（n-HA）及其与聚己内酯（PCL）的复合材料. 用熔融浇铸/食盐微粒浸出法制备了孔径在200～400μｍ、大孔互相贯通的复合材料支架. 通过细胞培养和体内动物实验研究了该支架的生物学性能. 结果表明，复合支架的孔隙率随致孔剂用量的增加而增加，而抗压强度随之而减小；支架的最大孔隙率可达86%，相应的抗压强度为2.4MPa. 成骨细胞在支架上的细胞粘附率和增殖随磷灰石含量增加而提高，复合材料明显高于单纯的PCL支架. 组织学观察显示，新生骨长入多孔支架和复合材料形成了直接的骨性结合. n-HA/PCL复合材料支架有很好的生物相容性和生物活性.
Nano hydroxyapatite (n-HA) and its composite with poly(ε-caprolactone) (PCL) were fabricated by hydrothermally synthesized method, and the well interconnected macroporous nano-composite scaffolds with pore diameter from 200μm to 400μm were prepared by melt-cast/salt particle-leaching technique. The biological properties of the composite scaffolds were investigated through cell culture and animal implanted experimentation. The results show that porosity of the composite scaffolds increases with the increase of the quantity of the porogens used while the compressive strength decrease. The maximal porosity of the composite scaffold can reach 86% while its compressive strength is only 2.4MPa. The attachment ratio and proliferation of MG63 cells on the composite scaffolds increase with the increase of HA content in the composite, which are significantly higher than those of PCL alone. Histological examinations confirm that the new bony tissue could grow easily into the composite scaffold and directly integrate with the composite by bone-bonding. The results indicate that the n-HA/PCL composite scaffolds have excellent biocompatibility and bioactivity.
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