C12A7-O-材料抗菌性能及机理研究
收稿日期: 2010-01-11
修回日期: 2010-02-24
网络出版日期: 2010-08-25
基金资助
国家“863”项目(2009AA05Z435); 国家“973”项目(2007CB210206); 国家自然科学基金(50772107)
Investigation on the Antibacterial Activity and Mechanism of C12A7-O- Particles
Received date: 2010-01-11
Revised date: 2010-02-24
Online published: 2010-08-25
使用气固反应方法制备了[Ca24Al28O64]4+·4O-(C12A7-O-)粉末材料, 运用X射线衍射(XRD)和电子自旋共振(ESR)对抗菌实验前后C12A7-O-材料结构特性的变化进行了表征, 研究了C12A7-O-材料在溶液中对大肠杆菌E. Coli的抗菌性能. 实验结果表明, C12A7-O-材料中含有的O-浓度约为2×1020 /cm3. 1 mg/mL的C12A7-O-水溶液中, O-浓度约为4×1016 /cm3. 当菌液浓度为105 cfu/mL时, 1 mg/mL的C12A7-O-水溶液可在1 h内将大肠杆菌浓度降低99.98%. 通过对比实验和分析发现, C12A7-O-粉末释放的O?离子是C12A7-O-溶液具有抗菌作用的重要因素之一.
宫 璐, 沈 静, 李全新 . C12A7-O-材料抗菌性能及机理研究[J]. 无机材料学报, 2010 , 25(9) : 942 -946 . DOI: 10.3724/SP.J.1077.2010.00942
The [Ca24Al28O64]4+·4O-(C12A7-O-) particle was firstly investigated its antibacterial activity against bacteria E. Coli in aqueous solution and phosphate buffered saline (PBS). The C12A7-O- particles were synthesized by gas-solid reaction method with CaCO3 and γ-Al2O3 in a molar ratio of 12:7. The structure change of the C12A7-O- particles was measured by X-ray diffraction (XRD) and electron spin resonance (ESR) after antibacterial test. The results show that the structure of the C12A7-O- particles is completely changed into the structure of (CaO)3Al2O3·6H2O and Al(OH)3. In the C12A7-O- solution (1 mg/mL), the concentration of atomic oxygen anion (O-) in the solution is 4×1016 cm-3. In the antibacterial test with the particle concentration of 3 mg/mL in aqueous solution, the bacteria concentration in decreased by 99.98% in 1 h from the original concentration of 105 cfu/mL. When the particle concentration is 1 mg/mL in PBS, the pH is 7.4 and the pH effect of the solution could be neglected, but the bacteria concentration still deduces to 3.7×103 cfu/mL from the original concentration in 24 h. In briefly, O- is the main factor in the antibacterial test in the C12A7-O- PBS solution with the neutral pH environment.
Key words： C12A7; antibacterial activity; antibacterial mechanism
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