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无机材料学报

无机材料学报

• 研究论文 • 上一篇    下一篇

β-磷酸三钙/聚L-乳酸与大鼠骨膜成骨细胞复合骨修复材料的研究

周大利1, 杨为中1, 尹光福1, 郑昌琼1, 陈锐2, 陈槐卿2   

  1. 1. 四川大学材料科学与工程学院, 成都 610064; 2. 四川大学生物医学工程研究室, 成都 610041
  • 收稿日期:2003-11-28 修回日期:2004-04-09 出版日期:2005-01-20 网络出版日期:2005-01-20

Characteristics of β-Tricalcium Phosphate/Poly (L-lactic acid) Composite Combining with Rat Periosteum Derived Osteoblast

ZHOU Da-Li1, YANG Wei-Zhong1, YIN Guang-Fu1, ZHENG Chang-Qiong1, CHEN Rui2, CHEN Huai-Qing2   

  1. 1. College of Material Science and Engineering, Sichuan University, Chengdu 610064, China; 2. Institute of Biomedical Engineering,
    Sichuan University, Chengdu 610041, China
  • Received:2003-11-28 Revised:2004-04-09 Published:2005-01-20 Online:2005-01-20

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摘要： 采用溶液浇铸-模压成型-沥滤方法制备了β-TCP/PLLA多孔支架材料, 将支架材料与大鼠骨膜成骨细胞复合获得新型组织工程骨修复材料. 通过抗压强度及压缩模量的表征研究了支架材料的力学性能; 采用SEM观测、MTT法、碱性磷酸酶活性及骨钙素分泌量检测细胞复合材料的体外成骨特性; 通过裸鼠肌袋种植, 以组织学方法评价细胞复合材料的异位成骨能力. 结果表明: β-TCP/PLLA多孔支架材料孔隙率可调, 孔径为100～00μm, 孔道相互贯通; 材料抗压强度和压缩模量随孔隙率的增大而降低, β-TCP复合PLLA后材料的力学性能高于同孔隙率的纯PLLA多孔材料; 复合支架材料适宜骨膜成骨细胞粘附和生长, 无细胞毒性; 骨膜成骨细胞复合β-TCP/PLLA支架材料的体外成骨特性良好, 且具有体内异位成骨能力.

关键词: β-磷酸三钙, 聚L-乳酸, 骨膜成骨细胞, 骨组织工程

Abstract: β-TCP/PLLA porous carrier material was prepared by the improved process consisting of solvent casting, compression molding and leaching. A novel tissue engineering composite was
developed by combining β-TCP/PLLA composite with rat periosteum derived osteoblasts. Mechanic properties of the composite carrier were determined
with compressive strength and compressive modular measuring; Osteogenesis characteristics in~ vitro were evaluated by means of SEM examination, MTT
assay, ALP activity and OCN content evaluation; Ectopic osteogenesis was investigated in muscle of nude mice by the histological method. Results show
that β-TCP/PLLA porous structure has adjustable porosity and interconnected pores with 100～200μm in size; Compressive strength
and compressive modulus of the materials decrease sharply with porosity increasing. Mechanic properties of β-TCP/PLLA in low porosity are
superior to those of PLLA alone with the same porosity; β-TCP/PLLA composite is fit for the adhesion and ingrowth of periosteum derived
osteoblasts, and has no cytotoxicity; β-TCP/PLLA composite combined with periosteum derived osteoblasts possesses good osteogenesis
potential in vitro and ectopic osteogenesis capacity in vivo.

Key words: β-tricalcium phosphate, poly L-lactic acid, periosteum derived osteoblast, bone tissue engineering

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