无机材料学报 ›› 2022, Vol. 37 ›› Issue (4): 427-435.DOI: 10.15541/jim20210264 CSTR: 32189.14.10.15541/jim20210264
所属专题: 【生物材料】骨骼与齿类组织修复(202409); 【制备方法】3D打印(202409)
收稿日期:
2021-04-19
修回日期:
2021-06-25
出版日期:
2022-04-20
网络出版日期:
2021-07-20
通讯作者:
朱敏, 副教授. E-mail: mzhu@usst.edu.cn;作者简介:
施吉翔(1991-), 男, 硕士研究生. E-mail: 929789873@qq.com
基金资助:
SHI Jixiang1(), ZHAI Dong2, ZHU Min1(), ZHU Yufang2()
Received:
2021-04-19
Revised:
2021-06-25
Published:
2022-04-20
Online:
2021-07-20
Contact:
ZHU Ming, associate professor. E-mail: mzhu@usst.edu.cn;About author:
SHI Jixiang (1991-), male, Master candidate. E-mail: 929789873@qq.com
Supported by:
摘要:
骨修复支架在植入缺损处后出现的炎症与氧化应激有关, 其中过氧化氢(H2O2)浓度过高是引起氧化应激的主要原因之一。二氧化锰(MnO2)能够通过催化分解H2O2来消除植入物周围环境过量的H2O2, 同时催化H2O2分解产生的氧气(O2)能够缓解骨缺损处因血供不足而导致的缺氧环境, 从而有利于骨组织再生与骨缺损修复。本研究采用简单的氧化还原法在3D打印制备的生物活性玻璃(BG)支架表面原位沉积MnO2颗粒, 得到BG-MnO2复合支架(BGM), 赋予BG支架清除H2O2的同时提供O2的能力。研究结果表明, BGM支架表面沉积MnO2含量随反应溶液中高锰酸钾浓度升高而增加, 其抗压强度随MnO2含量增加而增强, 但这些支架的孔隙率和降解速度基本保持不变。更为重要的是, BGM支架能够在H2O2环境中持续催化分解H2O2产生O2, 当不同Mn含量的BGM (BGM5和BMG9)支架在浓度为2 mmol/L的H2O2溶液中催化分解H2O2产生的O2能使溶液中饱和氧浓度分别达到8.4和11 mg/L。细胞实验结果表明, BGM支架对骨髓间充质干细胞的增殖和碱性磷酸酶活性有一定促进作用。因此, BGM支架在骨组织修复领域具有较大的应用潜力。
中图分类号:
施吉翔, 翟东, 朱敏, 朱钰方. 生物活性玻璃-二氧化锰复合支架的制备与表征[J]. 无机材料学报, 2022, 37(4): 427-435.
SHI Jixiang, ZHAI Dong, ZHU Min, ZHU Yufang. Preparation and Characterization of Bioactive Glass-Manganese Dioxide Composite Scaffolds[J]. Journal of Inorganic Materials, 2022, 37(4): 427-435.
图2 BG和BGM支架的(A1, A2, B1, B2, C1, C2, D1, D2) SEM照片和相应的(A3, B3, C3, D3)EDS图谱
Fig. 2 (A1, A2, B1, B2, C1, C2, D1, D2) SEM images and corresponding (A3, B3, C3, D3) EDS spectra of BG and BGM scaffolds (A1, A2, A3) BG scaffold; (B1, B2, B3) BGM1 scaffold; (C1, C2, C3) BGM5 scaffold; (D1, D2, D3) BGM9 scaffold
图5 (A)BG和BGM支架在Tris-HCl中浸泡28 d的降解曲线及(B)BG和BGM支架在SBF中浸泡14 d的pH变化曲线
Fig. 5 (A) Degradation curves of BG and BGM scaffolds in Tris-HCl for 28 d, and (B) pH change curves of SBF after BG and BGM scaffolds soaking for 14 d
图6 (A)BGM9支架在不同浓度H2O2溶液中的溶解氧水平和(B)BGM支架在 2 mmol/L H2O2溶液中溶解氧变化曲线(连续循环测试3次)
Fig. 6 (A) Dissolved oxygen levels in different concentrations of H2O2 solution after immersing BGM9 scaffolds, and (B) dissolved oxygen change curves in 2 mmol/L H2O2 solutions after immersing BGM scaffolds (cycle test for 3 times)
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