镍与绿茶EGCG对舌鳞癌细胞毒性的影响

doi:10.3724/SP.J.1077.2012.00179

无机材料学报 ›› 2012, Vol. 27 ›› Issue (2): 179-184.DOI: 10.3724/SP.J.1077.2012.00179 CSTR: 32189.14.SP.J.1077.2012.00179

镍与绿茶EGCG对舌鳞癌细胞毒性的影响

罗真兰, 王海波, 苏俭生

(同济大学口腔医学院, 上海 200072)

收稿日期:2011-02-27 修回日期:2011-05-17 出版日期:2012-02-10 网络出版日期:2012-01-05
作者简介:罗真兰(1983-), 女, 硕士. E-mail: zhenlanluo@sina.com
基金资助:
国家自然科学基金(30840031, 30970726) National Natural Science Foundation of China (30840031, 30970726)

Effect of Interaction of Ni²⁺ and Green Tea Epigallocatechin Gallate on Tongue Carcinoma Cells

LUO Zhen-Lan, WANG Hai-Bo, SU Jian-Sheng

(School of Stomatology, Tongji University, Shanghai 200072, China)

Received:2011-02-27 Revised:2011-05-17 Published:2012-02-10 Online:2012-01-05
About author:LUO Zhen-Lan. E-mail: zhenlanluo@sina.com

摘要/Abstract

摘要：

Ni²⁺是镍基牙科铸造合金中析出的主要金属离子, 是人体的致癌物质. 绿茶的主要活性成分表没食子儿茶素没食子酸酯(EGCG)对包括口腔癌在内的人体癌有化学预防作用. 本研究采用LC/MS法分析EGCG与Ni²⁺间的相互作用. MTT法检测EGCG、Ni²⁺及其相互作用对舌鳞癌细胞生长的影响. LC/MS分析显示, Ni²⁺与EGCG同时加入时有[EGCG-2Ni²⁺]和[EGCG-Ni²⁺]形成. Ni²⁺和EGCG在一定范围内对舌鳞癌细胞生长的抑制呈时间及浓度依赖性. EGCG与Ni²⁺共同作用后可明显增强对舌鳞癌细胞的抑制作用, 且抑制作用与它们的浓度、加入的顺序及Ni²⁺与EGCG的比例有关.

关键词: 牙科铸造合金, 绿茶, Ni²⁺, EGCG, 舌鳞癌细胞

Abstract:

Ni²⁺ is the major ion release from nickel-based dental casting alloy and carcinogens. The major polyphenolic constituent present in green tea, (-)epigallocatechin-3-gallate (EGCG), shows chemoprevention on human cancer. The aim of this study was to determine the interaction between EGCG and Ni²⁺ and the biological effects on tongue squamous cell carcinoma. The interaction of EGCG and Ni²⁺ was analyzed by LC/MS. The cell viability was assessed by the MTT reduction assay. The results of LC/MS indicated that [EGCG-2Ni²⁺] and [EGCG-Ni²⁺] formed when EGCG and Ni²⁺ were added simultaneously. Ni²⁺ and EGCG both inhibited the proliferation of tongue carcinoma cells in a time- and dose-dependent manner. The cytotoxicity against tongue carcinoma cells was increased when EGCG and Ni²⁺ cotreated, and the inhibition effects were extremely dependent on the concentrations of EGCG and Ni²⁺, addition order and the ratio of [Ni²⁺] to [EGCG].

Key words: dental casting alloy, green tea, Ni²⁺, EGCG: tongue carcinoma cells

中图分类号:

R783

罗真兰, 王海波, 苏俭生. 镍与绿茶EGCG对舌鳞癌细胞毒性的影响[J]. 无机材料学报, 2012, 27(2): 179-184.

LUO Zhen-Lan, WANG Hai-Bo, SU Jian-Sheng. Effect of Interaction of Ni²⁺ and Green Tea Epigallocatechin Gallate on Tongue Carcinoma Cells[J]. Journal of Inorganic Materials, 2012, 27(2): 179-184.

图/表 7

图1 负离子模式LC/MS分析EGCG与Ni2+间的相互作用

Fig. 1 LC/MS analysis of the interaction between Ni2+ and EGCG in the negative ion mode

图2 EGCG与Ni2+络合结构

Fig. 2 Construction of complexes of EGCG and Ni2+

图3 EGCG浓度对舌鳞癌细胞存活率的影响

Fig. 3 Cytotoxicity of EGCG concentration(50-500 μmol/L) against tongue carcinoma cells

图4 Ni2+浓度对舌鳞癌细胞存活率的影响

Fig. 4 Cytotoxicity of Ni2+ concentration(50-1000 mol/L) against tongue carcinoma cells

图5 EGCG与Ni2+共同处理舌鳞癌细胞48 h后的细胞存活率

Fig. 5 Effects of EGCG and Ni2+ concentrations on the cytotoxicity of EGCG against tongue carcinoma cells

图6 EGCG与Ni2+不同加入顺序对舌鳞癌细胞存活率的影响

Fig. 6 Effect of the addition order of EGCG and Ni2+ on the viability of tongue carcinoma cells

图7 Ni2+与EGCG(100 μmol/L)不同比例对舌鳞癌细胞存活率的影响

Fig. 7 Effects of the ratio of Ni2+ to EGCG on the viability of tongue carcinoma cells cells

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镍与绿茶EGCG对舌鳞癌细胞毒性的影响

Effect of Interaction of Ni²⁺ and Green Tea Epigallocatechin Gallate on Tongue Carcinoma Cells

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镍与绿茶EGCG对舌鳞癌细胞毒性的影响

Effect of Interaction of Ni2+ and Green Tea Epigallocatechin Gallate on Tongue Carcinoma Cells

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Effect of Interaction of Ni²⁺ and Green Tea Epigallocatechin Gallate on Tongue Carcinoma Cells