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无机材料学报

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2023 , Vol. 38 >Issue 12: 1457 - 1565

DOI: https://doi.org/10.15541/jim20230242

研究论文

氧化钛涂层润湿性对免疫成骨性能的影响规律

  • 上官丽 ,
  • 聂晓双 ,
  • 叶奎材 ,
  • 崔苑苑 ,
  • 乔玉琴
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  • 1.上海大学 材料科学与工程学院, 上海 200444
    2.中国科学院 上海硅酸盐研究所, 高性能陶瓷与超微结构国家重点实验室, 上海 200050
    3.中国科学院大学 材料科学与光电工程研究中心, 北京 100049
    4.上海大学 生命科学学院, 200444
上官丽(1997-), 女, 硕士研究生. E-mail: 20724695@shu.edu.cn
乔玉琴, 副研究员. E-mail: qiaoyq@mail.sic.ac.cn;
崔苑苑, 副教授, E-mail: cui-yy@shu.edu.cn

收稿日期: 2023-05-19

  修回日期: 2023-06-16

  网络出版日期: 2023-06-28

基金资助

国家自然科学基金(32171344);上海自然科学基金面上项目(20ZR1465100);上海市生物医药科技支撑专项(20S31903300)

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Effects of Surface Wettability of Titanium Oxide Coatings on Osteoimmunomodulatory Properties

  • SHANGGUAN Li ,
  • NIE Xiaoshuang ,
  • YE Kuicai ,
  • CUI Yuanyuan ,
  • QIAO Yuqin
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  • 1. School of Materials Science and Engineering, Shanghai University, Shanghai 200444, China
    2. State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China
    3. Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China
    4. School of Life Sciences, Shanghai University, Shanghai 200444, China
SHANGGUAN Li (1997-), female, Master candidate. E-mail: 20724695@shu.edu.cn
QIAO Yuqin, associate professor. E-mail: qiaoyq@mail.sic.ac.cn;
CUI Yuanyuan, associate professor. E-mail: cui-yy@shu.edu.cn

Received date: 2023-05-19

  Revised date: 2023-06-16

  Online published: 2023-06-28

Supported by

National Natural Science Foundation of China(32171344);Shanghai Natural Science Foundation(20ZR1465100);Shanghai Science and Technology Pillar Program for Biomedicine(20S31903300)

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摘要

钛及其合金以其优异的机械性能和生物相容性而被广泛应用作硬组织植入器械, 但其表面缺乏生物活性以及植入后的炎症反应易导致骨整合不佳。本研究利用不同气氛中的热处理工艺调控氧化钛涂层的润湿性, 并研究表面润湿性能对免疫反应和成骨性能的影响规律。研究结果表明, 与亲水(接触角~10º)的氧化钛涂层相比, 处于亲水/疏水临界状态的氧化钛涂层(接触角’90º), 在仅培养巨噬细胞时, 抑制了巨噬细胞向M1促炎方向极化; 在共培养小鼠骨髓间充质干细胞和巨噬细胞时, 促进了巨噬细胞向M2抗炎方向极化, 同时显著上调了骨髓间充质干细胞成骨相关标记物的基因表达, 显示出更好的免疫促成骨性能。

关键词： 表面润湿性; 氧化钛涂层; 退火; 免疫成骨

本文引用格式

上官丽 , 聂晓双 , 叶奎材 , 崔苑苑 , 乔玉琴 . 氧化钛涂层润湿性对免疫成骨性能的影响规律[J]. 无机材料学报, 2023 , 38(12) : 1457 -1565 . DOI: 10.15541/jim20230242

Abstract

Titanium and its alloys have been widely used as hard tissue implants due to their excellent mechanical properties and biocompatibilities. However, the lack of biological activity on its surface and the inflammatory reaction after implantation can easily lead to unsatisfactory osseointegration. In this work, the wettability of titanium oxide coatings was modulated by annealing in different atmospheres, and the effects of surface wettability on polarization of macrophages and osteogenic differentiation of mBMSCs were studied. The results showed that, compared to the hydrophilic titanium oxide coating (~10º, PEO-A), the titanium oxide coating with contact angle about 90º (PEO-A-V) inhibited the polarization of macrophages towards M1 pro-inflammatory direction under the mono-culture condition. However, under the co-culture condition, the titanium oxide coating with contact angle about 90º promoted macrophage polarization towards M2 and significantly upregulated gene expressions of osteogenic markers related to mBMSCs, indicating better immunomodulatory effects on osteogenic differentiation of mBMSCs.

Key words： surface wettability; titanium oxide coating; annealing; osteoimmunomodulatory effect

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