为了评价不同形貌的纳米羟基磷灰石粒子(nano-HA)对脑胶质母瘤细胞系U87细胞的作用，合成了三种不同形貌的nano-HA，与U87细胞共培养，培养基中同时还添加了聚乙二醇(PEG)，以保持nano-HA的稳定性。透射电镜(TEM)及动态光学散射(DLS)测定高度分散在培养基中nano-HA的稳定情况和粒子大小，MTT法检测了nano-HA对U87细胞的作用。实验结果表明，nano-HA可显著抑制U87细胞增殖；并且，针状纳米粒子的抑制能力要强于另外两种粒子。此外，通过RT-PCR检测发现不同形貌的nano-HA可不同程度的下调细胞的Toll样受体4(TLR4)的表达水平，这可能是其具有不同生物学活性的原因之一。然而，二者之间的具体关系和作用机制，尚需进一步的研究证实。
田 昂
,
王 超
,
薛向欣
,
吴安华
,
管格非
,
王 丽
,
仇 波
. 纳米羟基磷灰石对人脑胶质母细胞瘤U87细胞活性抑制效应的研[J]. 无机材料学报, 2010
, 25(1)
: 101
-106
.
DOI: 10.3724/sp.j.1077.2009.09246
To evaluate the effects of Hydroxyapatite nano-particles (nano-HA) with different morphologies on human U87 glioblastoma cell line (U87), three kinds of nano-HA were designed and synthesized, and a co-culture system containing nano-HA and U87 cells was established. Polyethylene glycol (PEG) was also added to the HA medium system for the samples stabilization. After determining the stabilization and high dispersed nanometer size of nano-HA in the medium system using Transmission electron microscope (TEM) and dynamic light scattering (DLS), the effects of nano-HA on GBM cells were assessed with an MTT method. Results showed that nano-HA could effectively inhibit U87 cells proliferation. Compared with other nano-HA, the needle-like nano-HA were more powerful in inhibiting U87 cells proliferation. Toll-like receptor 4 (TLR4) induced by different nano-HA may be one reason for their different bioactivity, however, the correlation between nano-HA and TLR4 expression needs further investigation.
[1]Bondy M L, Scheurer M E, Malmer B, et al. Cancer, 2008.113(7):1953-1968.
[2]Stupp R, Mason WP, Van Den Bent MJ, et al. N. Engl. J. Med, 2005.352 (10):987-996.
[3]Bauer I W, Li S P, Han Y C, et al. J. Mater. Sci: Mater Med, 2008.19(3):1091-1095.
[4]Murugan R, Ramakrishna S. Compos. Sci. Technol, 2005.65(15-16):2385-2406
[5]Li J , Yin Y, Yao F, et al. Mater. Lett, 2008.62(17-18): 3220-3223.
[6]Fu Q, Zhou N, Huang W H, et al. J. Biomed. Mater. Res. A, 2005.74(2):156-163.
[7]Yin M Z, Han Y C, Bauer I W, et al. Biomed. Mater, 2006.1(1): 38-41.
[8]Shi Z L, Huang X, Cai Y, et al. Acta. Biomaterialia, 2009.5(1):338-345.
[9]Cai Y R, Liu Y K, Yan W Q, et al. J. Mater. Chem, 2007.17(36):3780-3787.
[10]Li B, Guo B, Fan H S, et al. Appl. Surf. Sci, 2008.255(2):357-360.
[11]Yu L, Chen S. Cancer. Immunol. Immunother, 2008.57(9):1271-1278.
[12]Guo X Y, Gough J E, Xiao P, et al. J. Biomed. Mater. Res. A, 2007.82A(4):1022-1032.
[13]Yousefpour M, Afshar A, Yang X D, et al. J. Ectroanal. Chem, 2006. 589(1):96–105.
[14]Ma M H, Ye W, Wang X X. Mater. Lett, 2008.62(23):3875-3877.
[15]Cengiz B, Gokce Y, Yildiz N, et al. Colloids. Surfaces. A, 2008.322(1-3):29-33.
[16]Yin M Z, Han Y C, Dai H L, et al. J. Wuhan. Univ. Technol, 2006.21(4):102-104.
[17]Grandjean-Laquerriere A, Laquerriere P, Laurent-Maquin D, et al. Biomaterials, 2004.25(28): 5921-5927.
[18]Grandjean-Laquerriere A, Laquerriere P, Guenounou M, et al. Biomaterials, 2005.26(15):2361-2369.
[19]Grandjean-Laquerriere A, Tabary O, Jacquot J, et al. Biomaterials, 2007.28(3):400-404.
[20]Kundu S D, Lee C, Billips B K, et al. Prostate, 2008.68(2):223-229.
[21]Jego G, Bataille R, Geffroy-Luseau A, et al. Leukemia, 2006.20(6):1130-1137.
[22]Chochi K, Ichikura T, Kinoshita M, et al. Clin. Cancer. Res, 2008.14(10):2909-2917.
