气体发泡剂—NaHCO3和C6H8O7对可注射多孔硼酸盐玻璃基骨水泥性能的影响

doi:10.15541/jim20160532

摘要/Abstract

摘要：

硼酸盐玻璃具有优异的生物相容性、降解性和骨传导性, 在骨组织修复领域受到一定的关注。目前, 硼酸盐玻璃的主要应用形式是支架和微球, 而有关硼酸盐玻璃基骨水泥的制备及性能研究却较少涉及。基于孔隙在骨组织修复材料工程领域中的重要作用, 本研究以NaHCO₃和柠檬酸为气体发泡剂制备可注射的多孔硼酸盐玻璃基骨水泥, 通过SEM、XRD和FTIR等方法表征其对骨水泥性能的影响。结果表明: 发泡骨水泥具有良好的注射性, 其注射率高达80%。SEM形貌照片显示骨水泥中大孔已被成功引入, 且孔隙连通, 其孔径介于10 µm到800 µm之间。浸泡结果表明, 发泡骨水泥失重率明显提高。PBS溶液中浸泡30 d后, 发泡骨水泥的失重率高达70%, 而非发泡的骨水泥的失重率只有50%。此外, XRD和FTIR的结果表明, 浸泡产物为羟基磷灰石(HA)。ATR的结果进一步证明了BBG和PBS溶液的反应机理, 表明BBGC中孔隙的引入大大加快了其降解, 促进了矿化反应的进行。

关键词: 硼酸盐玻璃基骨水泥, 气体发泡, 大孔, 快速降解

Abstract:

Borate bioactive glass (BBG) is a promising candidate material for bone tissue engineering. Mostly, it was applied in the form of scaffolds and granules. Till now, little work has been done to investigate the fabrication of porous borate bioactive glass cement (BBGC). In this work, we prepared an injectable macroporous BBGC via gas-foaming method, where NaHCO₃ and citric acid were used as porogen. Porous structure was analyzed by using SEM. The properties of soaking products were characterized by XRD, FTIR and ATR. The injection test showed that the injection rate of BBGC was over 80%. SEM images presented that there were apparent pores in BBGC. The diameter of the macropores varied from 10 μm to 800 μm. Furthermore, the degradability of BBGC was greatly improved. After one month in vitro soaking, the weight loss of non-foamed BBGC was only 50wt%, while for the foamed samples, their weight loss reached 70wt%. Meanwhile, the XRD, FTIR and ATR results exhibited that there were hydroxyapatite (HA) formation and confirmed that the introduction of macropores into the BBGC did facilitate the mineralization reaction.

Key words: gas-foaming, bioactive borate glass cement (BBGC), macropores, fast-degrading

中图分类号:

TQ171

吴莹莹, 叶松, 姚爱华, 李海滨, 贾伟涛, 黄文旵, 王德平. 气体发泡剂—NaHCO₃和C₆H₈O₇对可注射多孔硼酸盐玻璃基骨水泥性能的影响[J]. 无机材料学报, 2017, 32(7): 777-784.

WU Ying-Ying, YE Song,YAO Ai-Hua, LI Hai-Bin, JIA Wei-Tao, HUANG Wen-Hai, WANG De-Ping. Effect of Gas-foaming Porogen-NaHCO₃ and Citric Acid on the Properties of Injectable Macroporous Borate Bioactive Glass Cement[J]. Journal of Inorganic Materials, 2017, 32(7): 777-784.

图/表 10

Table 1 Composition of the samples

Samples	S1	S2	S3	S4	S5	S6
BBG /g	1.5	1.5	1.5	1.5	1.5	1.5
(NaHCO₃/ BBG) / %	0	5	8	10	12	15
Gelatin solution / mL	1.0	1.0	1.0	1.0	1.0	1.0
Citric acid / mL	0	0.2	0.2	0.2	0.2	0.2

Fig. 1 Injectability study of the cements(a) Representative injectability curves of BBGC with different porogen contents obtained from a syringe with a 1.7 mm diameter at a compression rate of 15 mm/min and maximum applied injection force of 100 N; (b) Injectability percentage measured for BBGC

Table 2 Physical results of the BBGC

No.	S1	S2	S3	S4	S5	S6
Setting time / min	110 ± 10	175 ± 15	82 ± 5	55 ± 5	25 ± 3	20 ± 2
Density / (g•cm^-3)	1.10 ± 0.04	1.02 ± 0.02	1.04 ± 0.04	1.05 ± 0.04	0.98 ± 0.01	1.07 ± 0.04
Total porosity / %	47.07 ± 2.01	50.71 ± 0.85	49.38 ± 1.74	48.96 ± 2.13	52.18 ± 0.68	47.46 ± 1.89

Fig. 2 Fracture surface morphologies of injectable BBGC after one day setting in water bath at 37℃(a), (c) S2; (b), (d) S4

Fig. 3 Disintegration resistance of porogen-loaded BBGC after shaking for various times at 180 r/min in phosphate-buffered saline (PBS) at 37℃

Fig. 4 Morphologies of injectable BBGC after soaking in PBS and shaking at 180 r/min for 24 h

Fig. 5 Weight loss (a) and pH of PBS solution as a function of immersion time at 37℃ (b) of BBGC

Fig. 6 XRD patterns of BBGC (a) and (c), S2 by immersing in PBS with different soaking time; (b) and (d) S1, S2, S4 after immersion in PBS for 30 d

Fig. 7 ATR spectra of S1 and S4 as a function of soaking time (a), (c) materials 2 mm from the surface of S1 and S4 cylinder, respectively; (b) (d) surface materials of S1 cylinder and S4 cylinder, respectively

Fig. 8 Schematic illustration showing the degradation mechanism of BBGC in PBS solution as a function of times; sectional morphology of BBGC before soaking (a); BBGC soaking for some time (b); end product (c)

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气体发泡剂—NaHCO₃和C₆H₈O₇对可注射多孔硼酸盐玻璃基骨水泥性能的影响

Effect of Gas-foaming Porogen-NaHCO₃ and Citric Acid on the Properties of Injectable Macroporous Borate Bioactive Glass Cement

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