上转换荧光探针辅助的光学成像技术在肿瘤显影中的应用研究进展

海热古·吐逊, 郭乐, 丁嘉仪, 周嘉琪, 张学良, 努尔尼沙·阿力甫

Research Progress of Optical Bioimaging Technology Assisted by Upconversion Fluorescence Probes in Tumor Imaging

HAIREGU Tuxun, GUO Le, DING Jiayi, ZHOU Jiaqi, ZHANG Xueliang, NUERNISHA Alifu

图8 UCNPs用于UCL/MRI双模态成像[66]

Fig. 8 Application of UCNPs in UCL/MRI dual-modality imaging[66] (a) In vivo T1-weighted MRI of kidneys of mice (as arrowed) before and after the intravenous administration of NaGdF4@poly-L-lysine (PLL) nanoparticles; (b) Chemical exchange saturation transfer (CEST) contrast difference map between pre/post injection following radio frequency (RF) irradiation at 3.0 μT, showing the kidney signal in color on the grayscale image to highlight the effect; (c) In vivo T1-weighted MRI of brain tumor (as arrowed) after the intravenous administration of NaGdF4@PLL nanoparticles; (d) CEST contrast difference map between pre/post injection at 3.0 μT, showing the brain ventricle signal in color on the grayscale image to highlight the effect; (e) Merged image of (c, d); (f) H&E staining of the brain tumor tissue; (g) Immunohistochemical staining of the brain tumor tissue, showing the positive expression of glial fibrillary acidic protein