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上转换荧光探针辅助的光学成像技术在肿瘤显影中的应用研究进展
海热古·吐逊1(), 郭乐2, 丁嘉仪2, 周嘉琪1, 张学良1, 努尔尼沙·阿力甫1()
Research Progress of Optical Bioimaging Technology Assisted by Upconversion Fluorescence Probes in Tumor Imaging
HAIREGU Tuxun1(), GUO Le2, DING Jiayi2, ZHOU Jiaqi1, ZHANG Xueliang1, NUERNISHA Alifu1()

图6. 免疫荧光标记细胞的超分辨成像[50]

Fig. 6. STExD (stimulated-emission induced excitation depletion) imaging of immunolabelled subcellular filaments[50]
(a) Fluorescence image under 740 nm Gaussian beam excitation and (b) differential interference contrast image of actin protein in HeLa cells immunolabeled with phalloidin conjugated NaYF4: Nd nanoparticles; (c, f) Magnified areas and (d, g) super-resolution images under co-irradiation with 740 nm Gaussian beam and 1064 nm donut-shaped beam from white-dotted squares in (a); (e, h) Analyses of line profiles, indicated with white arrows in (c, d), as well as in (f, g), respectively; (i) Photon counts recorded with and without co-irradiation of 1064 nm donut-shaped beam with insets showing the corresponding images with the measured positions marked with white crosses