微纳米结构生物材料在骨组织再生修复中的研究进展

赵睿, 毛飞, 钱晖, 杨晓, 朱向东, 张兴栋

Micro-/Nano-structured Biomaterials for Bone Regeneration: New Progress

ZHAO Rui, MAO Fei, QIAN Hui, YANG Xiao, ZHU Xiangdong, ZHANG Xingdong

表1 微纳米结构生物材料用于成骨研究的文献总结

Table 1 Summary of previous work on bone formation in the micro-/nano-structured biomaterials

Material	Synthesis method	In vitro results	Animal model	In vivo results	Ref.
β-TCP scaffolds with micro/ nano surface topography	DLP printing and in situ growth crystal process	Promote osteogenic differentiation of stem cells	Rat skull defects	Improve the bone regeneration	[17]
Micro/nano-scale titania fiber-like network on the surface of Ti implants	One-step alkaline treatment in NaOH solution	Facilitate osteogenic and angiogenic differentiation of BMSCs and endothelial cells; Suppress M1 macrophages and stimulate M2 phenotype	Rabbit femur defects	Induce ameliorative osseointegration	[21]
MNBG/PLGA bi-layered membranes	Electrospinning	Promote osteogenesis			[24]
Micro-nano rough Ti₆Al₄V	Acid etch process	Improve osteogenic differentiation of MSCs			[26]
HA bioceramics with submicron- to nano- topographies	Sintering	Maintain the conformation of BMP-2, activate the osteogenic differentiation of BMSCs	Canine intramuscular implantation	Process excellent bone-like apatite forming ability and outstanding osteoinductivity	[28]
HA with micro/nano hierarchical structures	Photolithography and hydrothermal techniques	Promote osteogenic differentiation of hBMSCs and angiogenic acticvity of HUVECs			[41]
β-TCP/CaSiO₃composite ceramics with micro/ nano-HAp the surface layer	3D bioplotting and hydrothermal treatment	Upregulate the cellular differentiation of mBMSCs and gene expression of HUVECs	Ectopic subcutaneous implantation at the back of rats	Promote capillary formation and bone augmentation	[45]
PEEK/CF/n-HA ternary biocomposite with micro/ nano-topographical surface	Oxygen plasma and sandblasting	Promote the proliferation and differentiation of MG-63 cells	Dog mandibles	Boost the osseointegration between implant and bone	[73]
Micro/nano structural silicon nitride and PEKK composite	Femtosecond laser ablation	Promote osteogenic differentiation of rBMSCs; Exhibit a greater bacteriostatic activity	Rabbit femur cavity defect	Promote osseointegration and bone repair	[75]
Silicate-based bioceramic with micro-nano surfaces and hollow channels	3D printing and hydrothermal treatment	Facilitate the attachment and proliferation of BMSCs	Rabbit femur defects	Boost the newly bone formation	[80]
PLLA/CS composite scaffold with micro/nano- fiber hierarchical structure	3D printing and thermally induced phase separation technology	Promote cell adhesion and proliferation			[87]